Aerobacter aerogenesによるIsoamylaseの生産 : (第4報)溶存酸素濃度による流加培養の制御とその解析

概要

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With respect to the isoamylase formation by Aerobacter aerogenes, characrteristic studies on feeding cultivation have been reported on an earlier paper. In the case of feeding cultivation, the formed isoamyase activity per unit cell weight was higher in comparison with the results of batch cultivation. But there were many points which need to be clarified in order to establish the optimal conditions for feeding cultivation, because the cell concentration in the broth increased with the feeding of the medium.In order to clarify these phenomena, the quantiative correlations between cell concentration, rate of carbon source consumption, and rate of oxygen consumption were studied on the basis of a theoretical analysis.In feeding cultivation experiments, feeding rates of the carbon source were controlled automatically to provide an unlimited oxygen supply for cell growth. The final cell concentration obtained by feeding cutivation was about three times higher than that from batch cultivation; the isoamylase activity per unit cell in feeding cultivation also had a higher value. Experimental results for the feeding rate and cell growth showed good agreement with the values based on theoretical analysis in this paper.
1972-08-25