Aerobacter aerogenes による isoamylase の生産 : (第2報) 回分培養における定量的結果

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The previous report described a strain of Aerobacter aerogenes isolated for the purpose of industrial isoamylase production, and it was found that only cell-bound type isoamylase was formed by the use (NH_4)_2SO_4 as nitrogen source, which is characteristic of this strain.This strain was able to form isoamylase by using maltose, pullulan or soluble starch as the carbon source of its culture.In this report, some experiments were performed in order to compare various carbon sources in regard to the quantitative rate equations of cell growth and of isoamylase formation. This starain was cultured in 1000 ml glass jar fermentor. In the cases of cultibations with maltose, pullulan and glucose as carbon sources at a constant pH, the cell growth was investigated at the logarithmic growth phase and those rate constants (k) were 0.63,0.40 and 0.33 (1/hr) for glucose, maltose and pullulan respectively. On the other hand. when soluble starch was used in in the medium, the rate equation obtained was more complex than simply logarithmic. It was a 2nd order equation, namely In(C_xC_<S0>)/(C_SC_<x0>)=(k'R/m)t.This rate constant was calculated on the basis of many experimental data, and its value(k') was 0.051 (1/mg-hr). The isoamylase activity per unit cell weight was conspicuous in the culture using soluble starch.This value was obtained from a quantitative equation, E_2=25C_x. Isoamylase formations by maltose and pullulan were compared with that by soluble starch, and their yields were 5% and 10% of that by soluble starch respectively. When isoamylase was formed in the cell bound type, a quantitaively stable isoamylase formation was recognized with this strain.From these results, it was concluded that this strain and these rate equations were applicable for industrial purposes.
社団法人日本生物工学会の論文
1970-10-25

Aerobacter aerogenes による isoamylase の生産 : (第2報) 回分培養における定量的結果

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