ろ床物膜より分離した優勢バクテリアの基質除去特性 : 充てん塔式ろ床法による有機性廃水の処理(第5報)

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Substrate removal properties of six dominant bacteria isolated from biological film were studied by investigating COD and bacterial biomass in a rotary shaken culture using synthetic wastewater consisiting of glucose, Polypepton and KH_2PO_4・Total COD (COD_T) of supernatant liquid after centrifugation was assumed to be composed of the following two components : COD_C, originating from carbohydrates (glucose in this study) and calculated from the following stoichiometric relation : mg/l COD_C=mg/l Glucose×(192/180) and COD_N supposed to mainly originate from nitrogenous substrates (Polypepton i this study) and obtained by subtracting COD_C from COD_T).Flavobacterium sp. and Alcaligenes sp. removed COD_N faster than COD_C. The removal rate of COD_C increased considerably after the removable COD_N was removed almost completely. Triphasic COD_T removal by these two kinds of bacterium was observed in the time-course of substrate consumption. On the contrary, Klebsiella sp. removed COD_C completely and at the fastest rate among the six bacteria studied. It was noted that the presence of glucose blocked COD_N removal until the former was compltely consumed. Arthrobacter sp. removed only COD_C during the logarithmic phase of growth and COD_N was not removed until the phase of negative growht acceleration. The removal rate of COD_C with Arthrobacter sp. was much slower than with Klebsiella sp. ; however that of COD_N was the fastest among the six bacteria. Pseudomonas sp. consumed COD_C gradually, but both COD_N and biomass remained almost constant throughout the cultivation period. Bacillus sp. was able to remove both COD_C and COD_N at considerably rapid rates. With mixed culture systems which were composed of heterogeneous bacterial populations, a cooperative effect was found in the substrate removal, hence both COD_C and COD_N decreased at greater rates than with any pure culture system.Together with the kowledge of the succession of dominant bacterial flora reported in the preceding paper, these results allow the following reasonable conclusion : bacterial floras in biological film are constantly composed of such complementary members because as much as possible of both the carbonaceous and nitrogenous compounds can be removed.
1980-07-25

ろ床物膜より分離した優勢バクテリアの基質除去特性 : 充てん塔式ろ床法による有機性廃水の処理(第5報)

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