Aerobacter aerogenesによるisoamylaseの生産 : (第3報)流加法の検討

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In the previous publication, it was reported that the optimal condition for the formation of intra-cellular isoamylase in a strain of Aerobacter aerogenes in batch cultiture was given by the culvation of cells in the medium containing 1.0 % of initial concentration of any carbohydrate as carbon source and by cessation of that cultivation at the phase of 5-6 mg/ml cells in culture broth.In other to obtain the increased amount of isoamylase per unit volume of culture broth, the experiments were carried out in the medium containing more than 1.0 % of carbon source. Results of these experiments indicated that the rate of oxgen supply to the medium acted as the rate limiting factor for the cell growth and the activity of isoamylase per unit weight of cells decreased in that condition in comparison with the cells cultivated in the medium containing 1.0 % of carbon source. Effect of the dissolved oxygen concentration of isoamylase had been studied by the cultivation of A. aerogenes on the media with strictly controlled dissolved oxygen concentration. Results of these experiments showed that the isoamylse formation per unit cell weight was independent form the dissolved oxygen concentration in the culture as far as the level of that did not act as a limiting factor on the rate of cell growth.By the results of foregoing experiments, the methods of the feeding cultivation were tested. Feeding cultures with constant level (1.0 ppm) of dissolved oxygen concentration were conducted by three different feeding rates of medium containing 10 % of carbon source. It was observed that the feeding culture with 4400 mg/l-hr of feeding rate was able to produce 12-13 mg of cells per ml of final broth, which was approximately equivalent to two times higher concentration of cells than that of the batchwise cultivation.The isoamylase activity of the final broth of the feeding culture was about 420 units per ml and this value correspond to 35 units per mg cells obtained. Thus, the feeding culture achieved about 2.5 times of the final yield of isoamylase in the conventional batchwise culture.
社団法人日本生物工学会の論文
1971-07-25

Aerobacter aerogenesによるisoamylaseの生産 : (第3報)流加法の検討

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