Aerobacter aerogenesによるIsoamylaseの生産 : (第6報)酸素移動速度を制御した連続培養の解析

概要

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Generally, Continuous culture is carried out by controllig the limiting growth substrate. For aerated continuous cultivation, an important factor is oxygen consumption accompanied by consumption of substrate.Therefore, the rate of oxygen transfer for cultivation equipment was able to act as a factor for operation. In this situation a theoretical analysis for the case of an oxygen transfer rate of equipment equal to the oxygen consumption rate of the broth was performed, and some quantitative relations between the oxygen transfer rate and the dilution rate were derived.Experiments on continuous cultivation were carried out with the oxygen consumption rate equal to the oxygen transfer rate of the equipment used. That is, the operation of the cultivation was controlled automatically to 1.0 ppm of dissolved oxygen concentration in the broth by means of feeding rate regulation. Theoretically, cell concentration in the broth must be independent of dilution rate, since this cultivation was carried out by controlling the supply rate of the substrate in response to the rate of oxygen transfer of equipment. It was found that the experimental results agree well with this theory.From the results of a consideration of the experimental conditions, it was concluded that the method for the control and operation of the cultivation reported in this paper were an excellent method for continuous cultivation.
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