91(P-99) カリウドバチ毒嚢に含まれる生理活性物質の構造(ポスター発表の部)

概要

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Solitary wasp venom has attracted much interest as a source of neurotoxic compounds. However, only a little is known about chemical components of solitary wasp venoms. Their solitary lifestyle makes it difficult to collect a sufficient amount of sample for biochemical analysis. In this study, we analyzed the venom extracts of the spider wasp Cyphononyx dorsalis using MALDI-MS, which turned out to be a rapid and sensitive means for micro-scale analysis. In order to determine amino acid sequences of novel peptides, "nested CID/PSD" consisting of ladder sequencing and CID/PSD of an intact and a ladder peptide was used as well as MS/MS and Edman degradation. The biological properties of these novel peptides were investigated by using synthetic specimens only after characterization of peptide sequences because bioassay-guided fractionation in a usual manner was difficult due to small sample amount. Thus, a wasp-kinin (Thr^6-bradykinin) and two novel peptides were characterized by Edman degradation and MS/MS analysis. This is the first example for any kinins to be found in Pompilidae wasp venoms. Moreover, two other novel peptides in minor fractions were determined by nested CID/PSD. A part of this study was supported by a grant from the Research for the Future Program from the Japan Society for the Promotion of Science (JSPS), and FAPESP.
天然有機化合物討論会の論文
2000-10-01