37 タンデムマススペクトルによる構造解析 : 海洋ポリエーテル化合物への応用(口頭発表の部)

概要

論文の詳細を見る
Recently, marine polyether compounds have attracted much attention because of their extremely potent bioactivities, For the structure elucidation of these polyether groups, two-dimensional nuclear magnetic resonance (2D-NMR) experiments have been regarded as the sole tool. Yet the NMR methodology alone is sometimes insufficient to elucidate the structure with a molecular weight over 2000Da, or those with repetitive structure units which give rise to heavy overlapping of NMR signals. Fast atom bombardment tandem mass spectrometry (FAB MS/MS) played an essential role in the structural determination of polyhydroxy-polyether compound which have repetitive or similar structure moieties. Negative-ion FAB spectra of these compounds are typically dominated by molecular-related ion species due to the presence of a negative charge moiety such as a sulfate ester group. Collision induced dissociation (CID) of these molecular-related ion species gave charge remote fragmentation patterns which were very useful for structural determination. Maitotoxin, with a molecular weight of 3422Da, is one of the most potent and complex marine toxins. Conventional negative-ion FAB CID MS/MS spectra could not show simplified and prominent product ions of maitotoxin. Because of the intensity of the precursor ion of molecular related ion species corresponding to the ^<12>C monoisotopic mass is less than that expected from the natural abundance of ^<13>C. However, the negative ion FAB CID MS/MS spectrum measured with reduced selectivity for precursor ions gave a series of prominent product ions for the determination of structure, despite the location of one of the sulfate esters being in the middle of the molecule. The interpretation of the CID spectrum agreed with the structure of maitotoxin.
天然有機化合物討論会の論文
1995-09-01

37 タンデムマススペクトルによる構造解析 : 海洋ポリエーテル化合物への応用(口頭発表の部)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク