32 スクアリン酸をチャージリモートフラグメンテーションのトリガーに利用する含窒素生理活性化合物のMS/MS構造解析(口頭発表の部)

概要

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Toxins in venom grants of wasps, spiders, or scorpions have attracted much attention because of their intriguing biological activities as neurotoxins. Studies for isolation and structure determination of the venom components are extensive. These toxins are composed of complex composites including peptides, arylethanolamines, and acylpolyamines. In a venom grant, only a small amount of toxins is available in the level of pico to nano mole. Structure determination of these components requires a highly sensitive analytical method. Our approach to this subject is the use of various types of MS. Herein, we wish to report a new methodology for the structure analysis of peptide by MS/MS based on the use of squaric acid as a charge-remote fragmentation (CRF) inducing functional group. Model peptides containing a squaryl group (Sq) in the N-termini were synthesized by condensation of a Sq amino acid or a Sq acid diester with a tetrapeptide. FAB/CID/MS of the Sq group-containing peptides exhibited clear CRF patterns. These are the first observation that the Sq group induced CRF. The successful application of the present method to bradykinin and angiotensin-I in a small scale (10 nmol) are also disclosed.
天然有機化合物討論会の論文
2001-09-01