77 大腸菌Re変異株リポ多糖の構造と合成研究

概要

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Lipopolysaccharide (LPS) located on the cell surface of gramnegative bacteria is the chemical entity of endotoxin which exhibits a variety of biological activities. In the present work, the chemical structure of LPS of E. coli Re mutant was studied which represents the most simple type of LPS consisting of 3-deoxy-D-manno-2-octulosonic acid (2-keto-3-deoxyoctonic acid, KDO) and a lipophilic part designated lipid A. The main component of the LPS was isolated chromatographically after removal of the O-acyl groups followed by peracetylation and methyl esterification. Chemical as well as 2D-NMR and mass spectroscopic study revealed its structure to be 2. By taking account of the structure of lipid A determined by our previous study, the total structure of E. coli Re LPS could be elucidated as 1. A new preparative method for KDO derivatives and synthetic approach to Re LPS will be also described.
天然有機化合物討論会の論文
1985-09-07