87(P83) リポタイコ酸画分中のサイトカイン誘導活性複合糖質の研究(ポスター発表の部)

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We have recently demonstrated that several minor components which amount to only 2-3 wt % of the lipoteichoic acid (LTA) fraction from Enterococcus hirae ATCC 9790 possess cytokine-inducing activity, whereas the major component of over 90 wt % has no activity. In the present paper, we report an improved separation procedure and structural analysis of both biologically active and inactive components. In order to obtain sufficient active components for their structural study, their rapid and efficient separation was achieved by using batch- and stepwise elution from Octyl Sepharose followed by anion exchange membrane chromatography. The chemical structure of the inactive major component was first elucidated. The products from 47% hydrofluoric acid (HF)-hydrolysis were isolated and characterized by means of NMR and MS. The phosphodiester linkage between the hydrolysis products were determined by NMR spectra of alkali-treated inactive component without HF degradation. The structure was deduced as shown in 2, which coincides with the structure proposed by Fischer for LTA. This so-called "LTA structure" thus proved to be not responsible for the cytokine-inducing activity. The immuno-stimulating bioactive components from LTA contained rhamnose or mannose in addition to the common constituents found in the above bio-inactive component and were resistant to HF degradation. The chemical structure of these active components is now being examined in details.
天然有機化合物討論会の論文
1996-09-02

87(P83) リポタイコ酸画分中のサイトカイン誘導活性複合糖質の研究(ポスター発表の部)

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