P-59 S型ならびにR型リポ多糖の遠心液液分配クロマトグラフィーによる高効率分画とそれらの生物活性(ポスター発表の部)

概要

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S- and R-form lipopolysaccharides (LPS), which are structurally classified depending on the presence and absence of O-antigen polysaccharide chains in LPS, were effectively separated one another from native mixtures of smooth-type Salmonella abortus equi, Salmonella friedenau and Citrobacter freundii LPSs by means of the centrifugal partition chromatography (CPC) with high reproducibility and recovery rate. An LLB-M model CPC apparatus (Sanki Engineering, Kyoto, Japan) and a solvent system consisted of n-butanol/ tetrahydrofuran/methanol/water (10/7/1/20,v/v) were used for separation. The CPC-separated S- and R-form LPSs were assessed for their abilities to induce the secretion of tumor necrosis factor-α (TNF-α) in murine macrophage-like J774.1 cells in the presence or absence of fetal bovine serum. LPS dose-response and time-kinetics studies showed that serum factor(s) regulated especially the onset of TNF-α secretion in stimulation with S-form LPS having the O-antigen polysaccharide. But the effect of serum was only marginally in stimulation with R-form LPS which lacks the polysaccharide. These results clearly indicated that the native LPS activates macrophages in both O-polysaccharide/serum-dependent and -independent pathways.
天然有機化合物討論会の論文
1994-09-20