18 グラム陽性連鎖球菌細胞表層のサイトカイン誘導活性複合糖質の研究(口頭発表の部)

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We have demonstrated that several minor components which amount to 2-3 wt % of the lipoteichoic acid (LTA) fraction from Enterococcus hirae ATCC 9790 possess cytokine-inducing activity, whereas the major component of over 90 wt % has no activity. The chemical structure of the major component coincides with the structure proposed for LTA by Fischer. In the present paper, we report structural analysis of a biologically active minor component in the LTA fraction and an improved separation procedure for active glycoconjugates from bacterial cells. The active minor component, designated OS-4L, from the LTA fraction was subjected to hydrolysis with aqueous HF to give a polysaccharide, low molecular weight hydrophilic products, and lipophilic products. The polysaccharide was composed mainly of highly branching mannan as determined by NMR and MS analyses. The structure was close to that of a yeast mannan, which, however, showed no cytokine-inducing activity. The low molecular weight products consisted of phosphate and glycerol, suggesting the presence of a glycerophosphate structure in OS-4L. The lipophilic products was not fully characterized because of its limited amount. By employing a more efficient separation procedure, in which active fractions were directly separated from a crude extract by anion exchange chromatography, several new active components different from above OS-4L were isolated from the same bacteria. On HF hydrolysis, the major new active components gave lipophilic products similar to those from above OS-4L, suggesting the importance of these lipophilic parts for the biological activity.
天然有機化合物討論会の論文
1998-08-31

18 グラム陽性連鎖球菌細胞表層のサイトカイン誘導活性複合糖質の研究(口頭発表の部)

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