遅延型アレルギーにおける補体の関与 : 第1報.脱感作による血中補体価の変動

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It is well known that serum complement level decreases markedly in anaphylactic shock. However, the role of complement is not so clear in delayed-type hypersensitivity. Therefore, serum complement activities were measured before and after desensitization to analyze the relationship between complement and delayed-type hypersensitivity. Materials and Methods: Guinea pigs were sensitized by an intramuscular injection of liquid paraffin emulsion containing 0.5 mg of heat killed tubercle bacilli (Aoyama B strain). Each of these animals showed a strong skin reaction to 0.1 ml of 2000 fold diluted old tuberculin (OT), i.e., more than 12 × 12 mm in redness and induration at 24 hours, when tested 8 weeks after the sensitization. These guinea pigs were divided into three groups. The first group was desensitized by an intravenous injection of PPD (Purified Protein Derivatives, 200 γ-4 mg). The second group was desensitized by an intraperitoneal injection of OT (1 mg of 10 fold diluted solutions to 1 ml of OT). The third group was desensitized by long term, gradually increased subcutaneous injections of OT. The guinea pig sera were taken before sensitization, just before, 1 hour after, 24 hours after and 48 hours after desensitization respectively. Following examinations were performed in each animal. 1. Fifty per cent hemolytic activity of complement (C'H50). 2. Fifty per cent immuno-adherence activity of complement (C'IA50). 3. Hemolytic antibody titer of Middlebrook-Dubos Reaction (M.D. titer). Results: 1. There was no significant difference in C'H50 between before and after sensitization. 2. Only slight reductions in C'H50 were observed in the guinea pigs desensitized by PPD. 3. Extremely marked reductions of C'H50 were noticed in the OT-desensitized group in accordance with the decrease of MD titer. 4. C'H50 almost recovered in 48 hours after desensitization. 5. C'H50 showed no significant changes in the gradually desensitized group. 6. C'IA50 changed well parallel with C'H50. Conclusions: The decrease in C'H50 was marked in the OT-densensitized animals in accordance with M.D. titers. While the changes were very slight in the PPD-desensitized. Therefore, the direct evidence of the significant role of serum complement was not obtained in delayed-type hypersensitivity.
日本アレルギー学会の論文
1968-01-30

遅延型アレルギーにおける補体の関与 : 第1報.脱感作による血中補体価の変動

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