69 Frit-FAB LC/MSを用いた担子菌生産抗生物質Glykeninの構造解析(ポスター発表の部)

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A strain of Basidiomycetes sp. produces a mixture of glycosidic antibiotics, glykenin (GK) complex, which exhibit inhibitory activity against gram-positive bacteria. They consist of three tetrahydroxylated C_<26> fatty acids (A, B, and C) as the aglycones and partially acetylated trisaccharides composed of a glucose and two xylose (Fig. 1), so that over twenty components are present in the GK complex. However, a normal phase TLC can separate only seven or eight components, in which GK-III and IV are major (Fig. 2). In order to separate and charactarize structurally each component in such complicated mixture, LC/MS was considered to be best suited and Frit-FAB LC/MS was applied according to the following sequence. The respective components were roughly isolated by silica gel column (Fig. 2) and were separated by HPLC with RI detection under optimized conditions. As shown in Fig. 4, each GK component was composed of three components containing the different aglycones (A, B, and C). Subsequently, each was applyed to Frit-FAB LC/MS and the resulting diagnostic ions are summarized in Table 1. The differenciation of the aglycone A from those of B and C can be performed using the aglycone derived ions, m/z 459, 285, and 299, and the location of acetyl groups in the sugar moieties can be elucidateted from the sugar related ions. The structures of GK components can be deduced by the Frit-FAB LC/MS and the profile analysis of the GK complex (Fig. 6) was successfully carried out under the optimized gradient conditions.
天然有機化合物討論会の論文
1992-09-10

69 Frit-FAB LC/MSを用いた担子菌生産抗生物質Glykeninの構造解析(ポスター発表の部)

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