37 高速向流クロマトグラフィーによる天然有機化合物の分離(口頭発表の部)

概要

論文の詳細を見る
Countercurrent chromatography (CCC) is a unique form of liquid-liquid partition chromatography that eliminates the need for a solid supporting matrix. Centrifugal devises, which comprise the majority of CCC apparatus, retain effectively the stationary phase in a helical column by gravitational or centrifugal force. Especially, high speed CCC (HSCCC) which has been extensively developed by Ito, one of the authors, is useful in the preparative-scale separation. In the present study, optimization of several parameters, selection of appropriate two-phase solvent system, stationary phase retention, revolution speed and flow rate of mobile phase, was carried out for obtaining satisfactory results. Finally HSCCC was successfully applied to the preparative-scale separation of components of sporaviridins (Fig. 1) and bacitracins (Fig. 2) under the optimized conditions. These results clearly suggest that HSCCC is promising for preparative separation of natural products and a combination of HSCCC and HPLC would greatly contribute to this field.
天然有機化合物討論会の論文
1988-09-26