アルミニウム製逆相TLCプレートの食用タール系色素分析への応用

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We have applied an aluminum-backed reversed-phase TLC plate to the analysis of the 12 coal tar dyes permitted to use for foods in Japan. Two solvent systems were selected for the separation of the xanthene dyes (methanol-acetonitrile-5% aqueous sodium sulfate solution=5:1:3) and the others (methanol-acetonitrile-5% aqueous sodium sulfate solution=3:3:10) (Fig.1). Fifty-four commercial foods were analyzed by aluminum-backed reversed-phase TLC after clean up of the dyes using polyamide column chromatography, and chromatographic behaviors of the dyes were observed. The average Ra/Rs values, ratios of Rf values of sample and standard spots on the same TLC plate, ranged from 0.99 to 1.08 and their coefficient of variation values were 0.0-5.1% (Table 1 and 2). These results mean that the separation was not affected by coexisting substances from foods and the spots always gave the same Rf values as the standards with good reproductibility. In TLC/fast-atom bombardment mass spectrometry (FABMS) of the coal tar dyes, use of aluminum-backed reversed-phase TLC plate improved the detection limits about 2-fold in comparison with use of glass-backed reversed-phase TLC plate except for azo dyes (Table 3). Finally, TLC/FABMS with the aluminum-backed plate has been successfully applied to the identification of Azo Rubine and Wool Green S, which are not permitted to use for foods in Japan, in the imported foods (Fig. 2). Therefore, aluminum-backed reversed-phase TLC plate is considered to be useful tool for the analysis of coal tar dyes in foods.
日本食品化学学会の論文
1998-11-30

アルミニウム製逆相TLCプレートの食用タール系色素分析への応用

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