88 天然物化学におけるクロマトグラフィー : 質量分析法の有効利用(口頭発表の部)

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Recent advance of spectroscopic instrumentation such as NMR spectroscopy has greatly facilitated structural determination of natural products, so that purification and isolation has become further important in this field. A combination of mass spectrometry and chromatography is being much paid attention for simultaneous separation and structural characterization of natural products. Fast atom bombardment mass spectrometry (FABMS) has contributed for obtaining molecular weight information of polar and involatile compounds. Recently, the use of a frit probe as an interface for FABMS analysis of HPLC effluents has been established. In this method effluent from HPLC was split with a pneumatic splitter or flow splitter and the resulting smaller portion was introduced into a frit surface via a fine capillary tube, followed by FAB ionization on the frit surface as a target (Fig. 2). The method was successfully applied to structural characterization of minor components of peptide antibiotic, bacitracin (BC) and the structures of 13 components were proposed as shown in Fig. 3. We have also developed another combination technique using TLC and FABMS. In order to perform effectively TLC/FABMS, a condensation technique was devised. Namely, methanol was applied around a developed spot on a TLC plate and then the sample was condensed to the center of the spot in line with penetration of methanol (Fig. 6). The method including the condensation technique has been applied to various natural products.
天然有機化合物討論会の論文
1991-09-07

88 天然物化学におけるクロマトグラフィー : 質量分析法の有効利用(口頭発表の部)

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