116(P-66) フィリピンで発生した致死性カタオゴノリGracilaria edulis食中毒原因物質の同定、ポリカバノシド類の起源、生理作用および新規類縁体の構造(ポスター発表の部)

概要

論文の詳細を見る
Outbreaks of seaweed poisonings are widely spread over the pacific area. Fatal glycosidic macrolides, polycavernosides, and potent tumor promoters, aplysiatoxins, have been previously isolated from edible seaweed. During 2002-2003, three fatal poisoning incidents caused by ingestion of two edible red alga, Acanthophora specifera and Gracilaria edulis, in Philippines causing eight deaths among 36 patients. We identified polycavemoside A from the causative G. edulis for 2 December 2002 event by LC-diode array detection and LC/electrospray-MS analyses. Poplycavernoside A was also identified in the attachment to this toxic alga by LC/MS/MS. Cyanobacteria attaching to this alga were speculated to be an origin of polycavernosides. We also investigated the effect of polycavernoside in cultured Neuro-2a cell. The synthetic polycavemoside analog, possessing a conjugate enynene side chain at C15 instead of a conjugate triene in polycavemoside A, induced apoptosis at the concentration of 12μM. In addition, we also elucidate the structures of two new minor polycavernoside congeners (PC and PC2) isolated from G. edulis collected in Guam in 1991 and 1992. According to the spectroscopic data, these compounds were suggested to possess a common aglycon, which is different from that of polycavernoside A.
天然有機化合物討論会の論文
2004-10-01