P-16 ヒガンフグFugu pardalisのサキシトキシン、テトロドトキシン結合蛋白質の抗体作製と分布解析(ポスター発表の部)

概要

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Puffer fish accumulate tetrodotoxin and saxitoxin in their tissues, mainly in eggs, livers, skin, and intestine. We previously isolated saxitoxin and tetrodotoxin binding protein (PSTBP) form plasma of the puffer fish, Fugu pardalis, cloned cDNA coding this protein, and clarified its binding properties to these toxins. PSTBP monomer consists of 42kDa protein part and 64kDa sugar part. We now prepared specific polyclonal antibody against PSTBP by immunization of rabbit by injection of the protein part of PSTBP as antigen obtained by expression of its cDNA in E. coli. Obtained anti-PSTBP antiserum and IgG purified from this antiserum were used to investigate localization of PSTBP in F. pardalis tissues by western blot analysis and immunohistochemical staining, respectively. PSTBP were detected in all tissues tested by western blotting analysis, and most prominently detected in skin.
天然有機化合物討論会の論文
2005-09-15