P-3 テトロドトキシン類縁体の生物内分布調査とヒガンフグ卵巣中の6,11-ジデオキシテトロドトキシンの単離(ポスター発表の部)

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The anatomical distribution of tetrodotoxin (TTX), saxitoxin (STX) and their analogs (TTXs, STXs) in three female and three male specimens of the marine puffer fish Fugu pardalis from Miyagi Prefecture, 2005, Japan, were studied. 5-DeoxyTTX, 11-deoxyTTX, and 5,6,11-trideoxyTTX were quantified by liquid chromatography/mass spectrometry (LC/MS) for the first time, and other TTXs and STXs were determined by liquid chromatography-fluorescent detection (LC-FLD). As a result, 5,6,11-tridoexyTTX was found to be the major TTX analog in all tissues tested, whereas 5-deoxyTTX and 11-deoxyTTX were minor components. Especially, in female (n=3), the ratios of 5,6,11-trideoxyTTX to total of all TTX analogs (mol/mol) in ovaries (mean±SD, 0.42±0.055) were significantly larger than those in livers (0.17±0.025)(P<0.05). In contrary, the ratios of 4,9-anhydroTTX to total of all TTX analogs in livers (0.27±0.047) were significantly larger than those in ovaries (0.073±0.040)(P<0.01). In male (n=3), all these ratios were not significantly different between livers and testis. 4-S-CysteinylTTX was detected in liver, spleen, gall, and intestine in 1-6 mol% of total of all TTX analogs, supporting our previous hypothesis that 4-S-cysteinylTTX was a metabolite of TTX. In addition, 6,11-dideoxyTTX was isolated from ovary of F. pardalis.
2006-09-15