68(P-25) 沖縄産ハブクラゲ(Chiropsalmus quadrigatus)刺胞毒の化学的性状(ポスター発表の部)

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The deadly box jellyfish (Sea Wasp) Chiropsalmus quadrigatus Haeckel (Cubozoa) is distributed widely in the tropical Pacific region. In Japan, three fatal cases due to stings from this species have been reported officially. We successfully isolated C. quadrigatus toxin-A (CqTX-A, 44kDa), a major proteinaceous toxin, for the first time, from the nematocysts of C. quadrigatus. CqTX-A showed lethal toxicity to crayfish when administered via intraperitoneal injection (LD_<50>=80_g/kg) and hemolytic activity toward 0.8% sheep red blood cells (ED_<50>=160 ng/kg). Furthermore, we sequenced the cDNA encoding CqTX-A. The deduced amino acid sequence of CqTX-A (462 amino acids) showed 25.2% and 21.6% homology to Carybdea rastoni toxins (CrTXs) and Carybdea alata toxin-A (CrTX-A), respectively, which are Cubozoan jellyfish toxins.
2001-09-01