麹菌の生産するプロテアーゼ II. : 酸性プロテアーゼの多様性の検討

概要

論文の詳細を見る
Acid protease fraction obtained from taka-diastase preparation was further fractionated into 3 to 4 fractions by means of column-chromatography using Amberlite XE-64.Rice-koji extract also was proved to contain 4 kinds of acid proteases by means of zone-electrophoresis at 0〜2℃ with starch as supporter. These fractions were named, resprctively, acid proteases II, Iα, Iβ and Iγ, referring to the results previously reported.In order to study the origine of such multiplicity, the separate fractions were again subjected to electrophoresis at pH 5.5,on one band, and the change in the composition of the acid protease fraction with incubation time was followed, on the other.It was indicated from the results that acid protease II is transformed into the enzymes of I group. At least, it was observed that acid protease Iα formed from II was transformed into Iβ. No protease activity was shown in the dialyzable fraction from autodigestion mixture of acid protease. Therefore, the observed transformation of acid proteases is considered to be similar to that of chymotrypsin.
公益社団法人日本生物工学会の論文
1961-04-15