カビ類の発生するβ-1,3-グルカナーセについて

概要

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Production and properties of fungal β1,3-glucanase were investigated employing three species of cellulase-producing fungi, Aspergillus niger U_<20> Penicillium variable and Trichoderma viride. Firstly, was dealt with relation of nutritional condition, especially of nitrogen sources, to the production of the enzyme in submerged culture(Table 1).Induced formation of the enzyme was effecterd by yeast glucan or laminarin as inducer; cellulose was without such effect (Table 2). Partially purified enzyme preparations were subjected to zone electrophoresis on starch gel (Fig. 2). The electrophoretic patterns showed two (Asp. nivger) or three (Pen. variabile and Trich. viride peaks of β-1,3-glucanase activity; all the fractions corresponding to these peaks yielded only glucose from yeast glucan even in an early stage of reaction, indicating tht these were of exo-glucanase type (Fig. 3).Optimum pH values of these enzymes were 2.5〜3.5 (Asp.niger) and 4 (Pen. and Trich.). Optimum Temperature for 1 hr-reaction was ca. 50℃, and stability optimum pH 4,in all instances presented. Contrary to the endo-glucanase of Streptomyces albiodoflavus, these fungal enzymes were shown to be almost incapable of lyzing yeast cell.
社団法人日本生物工学会の論文
1965-10-25