酵母細胞壁溶解酵素にかんする研究 : (第3報) プロテアーゼ活性区分について

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As reported previously, yeast cell wall-lytic enzyme system could be divided into two, β-1,3-glucanase and protease, fractions; each fraction had the cell wall-lytic activity.In this report, the latter fraction (P-fraction) was investigated in an effort to elucidate the relationship between proteolytic and cell wall-lytic activities thereof and to make comparison with "Pronase".(1) Some differences with regard to the activity-pH, stability-pH and stability-temperature relationships were observed between proteolytic and cell wall-lytic activities as shown in Fig. 1〜3. One of the remarkable difference, for instance, was shown in pH optima, i.e. pH 8.5 for protease activity and pH 7〜8 for cell wall-lytic acticity.(2) Both of the above-mentioned activities were very sensitive to potato inhibitor(PI) and almost insensitive to EDTA. On the other hand, protease activity of "Pronase"was sensitive to EDTA and slightly sensitive to PI, while its cell wall-lytic activity was inihibited not by EDTA but by PI (Table 1).(3) Zone-electrophoretic patterns of P-fraction of starch gel showed two peaks for cell wall-lytic activity and one for protease activity. But the shoulders, of protease and lytic activity curves being covered by the peaks of lytic and protease activities, respectively, seemed to indicate that all these fractions had the two activities, though the proportions of these activities were different from each other (Fig. 4; Table 2).Tests for peptidase activities with various synthetic peptides as substrates revealed the difference in specificity among these fractions (Table 3).(4) Electrophoretic pattern of "Pronase" showed two peaks : one was of protease activity and the other cell wall-lytic activity, and the shoulder of the protease activity curve was overlapped by the lytic activity peak (Fig. 5).(5) Natural proteins, such as casein, egg albumin and soybean protein, were hydrolyzed by the crude cell wall-lytic enzyme preparation into amino acids in a good yield;maximum degrees of hydrolysis lay between 80 and 85% (Table 4).
社団法人日本生物工学会の論文
1965-10-25

酵母細胞壁溶解酵素にかんする研究 : (第3報) プロテアーゼ活性区分について

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