酵母細胞壁溶解酵素にかんする研究 (第1報) : 菌株の分離と酵素生産条件の検討

概要

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1) About a hundred strains capable of forming clear zones around colonies grown on a nutrient agar plate, in which crude preparation of yeast cell wall was finely distributed, were isolated from soil samples. Out of these strains, a strain (A-14) was selected by tests lytic activities, toward heat-treated yeast cells, of the filtrates from shaken cultures.According to the Waksman's system of taxonomy, the strain was shown to be closely related to Streptomyces albidoflavus.2) Quantitative expressions were made of the lytic effect (LE) and the lytic activity (LA) based on the decrease in optical desity of a suspension of fresh or treated cells.3) Yeast glucan was shown to be a very effective inducer for the emergence of lytic activity.Presumably, β-glucanase whose action on yeast cell wall was shown to be accelerated synergistically by Streptomyces protease(presented at the Annual Meeting of Agric. Chem. Soc. Jap., April, 1962), was induced in this case.4) Nitrate as nitrogen source was conspicuously effective in raising the lytic activity of culture fluid when applied in conjunction with an appropriate organic nitrogen source and inducer source.
社団法人日本生物工学会の論文
1962-07-15