Glucamylase生産におけるPreferential Synthesisの生理学的解明とその多段連続培養への応用 (第1報)

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1. An adenine-requiring strain (U20-2-5) of Aspergillus niger has been employed taking advantage of the fact that is growth rate and the maximum level of growth can be controlled by the concentration of adenine-group substances including adenine, adenosine, ATP, hypoxanthine, inosine, inosinic acid and purine.2. The time-course of metabolism in a batch culture is composed of two phases, namely proliferating phase (earlier phase) and glucamylase-producing phase(later phase), indicating that it belongs to the type of preferential synthesis. Ordinarily, the former phase terminates with teh shortage of adenine-group substances and is followed by the latter. Experiments with a number of substances efective for enzyme production have shown taht guanine, guanosine, guanilic acid and xanthine (guanine-group substances) are effective in accelerating enzyme formation. These guanine-group substances cannot be substituted for adenine-group substances as growth factors. Based on the above, a metabolic map of purine-interconversion in this strain has been depicted, in which the step, GMP to IMP, is presumed to be lacking.3. The relationship of specific growth rate to adenine concentration has been revelated to be in conformity with the Monod's equation, in which the Ks-value of approximately 37μM is obtained by the plot of Lineweaver-Burk.4. In the glucamylase-producing phase, the release of the enzyme pre-existent in mycelia is not significant; the enzyme is produced under conditions where active energy metabolism is going on. In anaerobic condition, in the presense of an effective amount of uncoupler (2,4-dinitrophenol) or of respiration inhibitors (azide and arsenate), amylase activity relased is not more than one-fifth of that normally produced in the parallel experiments.5. Guanine is effective also in proliferating phase in that it contributes to the enzyme producing activity of mycelia made up in its presence, which is exerted in the later phase.6. Results of replacement culture have indicated that the filtrate from a culture in the enzyme-producing phase in much more suitable for enzyme production that in the earlier phase.Also the mycelia hervested from the later phase, which has been metabolizing in the adenine-deficient condition, are much more active in enzyme production than those from the eariler phase of culture when replaced to the same medium adequate for enzyme production.7. The above results are considered to furnish informations important for the biochemical control of multi-stage continuous fermentation with cultures belonging to the type of preferential synthesis.
社団法人日本生物工学会の論文
1963-05-15

Glucamylase生産におけるPreferential Synthesisの生理学的解明とその多段連続培養への応用 (第1報)

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