Carbon Column Chromatography による糖類の分離に就いて

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This paper deals with the behavior of sugars in charcoal-celite column chromatography. Glucose, maltose and maltotriose were used separately and in combination. The adsorbent consisted of equal parts by weight (15g) of Shirosagi charcoal (TAKEDA Co.) and celite.For the elution of the adsorbed sugars, water and ethanol were used in succession, and the eluates were collected in each 25m l fraction. The course of elution was tested by the MOLISCH reaction, and the eluted sugars in each fraction were determined after preliminary detection by paper chromatography.In all cases, glucose was completely eluted with water, but the rate of elution varied to some extent with the sugar concentration in the solutions used for the adsorption.Maltose was not completely eluted with water, but by succeeding treatment of the column with dilute ethanol, the recovery of maltose increased considerably, and it attained to almost 100%, when the sugar concentration in the original solution was 1%.The mixture of glucose and maltose was separated in the same manner. In all cases, almost complete elution of glucose was attained by sucking water and dilute ethanol in succession through the column. But a small amount of glucose was always detected by paper chromatography in the initial fractions of maltose. The mixture of glucose, maltose and maltotriose was also separated in the same manner.
社団法人日本生物工学会の論文
1958-09-15