リンゴの加工に關する研究 (第4報) : ペーパークロマトグラフ法による果汁成分の検索に就いて

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Sugars, organic acids, and amino acids of apple juice were detected by paper partition chromatography, and simultaneously these components were determined in usual way.1) The sugar content of apple juice was as follows : Total sugar (calculated as invert sugar)11〜14.5 g/100ml, the ratio of glucose : sucrose : fructose 1 : 1.2〜2.8 : 1.7〜3.5. And on the chromatgram, 4 spots corresponding to glucose, fructose, sucrose, and xylose were observed. Xylose was not nitherto detected in apple juice.2) The organic acid of apple juice was 0.18〜0.62 g/100ml (Ph 3.4〜4.2), and on the chromatogram only one spot was recognized, which corresponds to malic acid.4) The amino acid nitrogen of apple juice was about 0.013 g/100ml, and by the chromatography of natural juice, aspartic acid, glutamic acid and asparagine were identified, but, when the amino acids in juice were separated by treating with Hg acetate, set free by H_2S, and concentrated, further amino acids : serine, glycine, valine, alanine, leucine or isoleucine, tyrosine and arginine were detected.
社団法人日本生物工学会の論文
1951-05-15