非醗酵性糖に關する研究(第2報) : 糸状菌酵素による非醗酵性糖の生成

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We studied the production of unfermentable sugars by action of fungal enzymes on maltose, glucose, starch, etc., and obtained the following results : (1) Filtrates from the fungal culture (Asp. niger NRRL 337,Asp. niger sp., Asp. oryzae sp., and Rhizopus javanicus T.) were added as fungal enzymes to the buffered maltose solutions and the reaction mixtures were incubated for 48〜96 hr at 55℃. We have demonstrated by paper partition chromatography that these filtrates synthesize unfermentable sugars (isomaltose, cellobiose, panose and 2 or 3 unknown sugars) from maltose and starch. The constituents of sugars which were synthesized differed according to the kind of molds.(2) Maltose spot which was recognized as maltose by one dimension paper chromatography, was divided into two spots by multiple chromatography. The sugars of these two spots were unfermentable by yeast and we observed that the one was cellobiose and the other was an undetermined disaccharide.(3) Taka-diastase synthesizes isomaltose and panose from maltose.(4) The filtrates from the fungal culture (Asp. niger NRRL 337,Asp. niger sp. and Asp. oryzae sp, ) and Taka-diastase synthesized isomaltose from glucose but not from arabinose, xylose, galactose, fructose, mannitol, sucrose and inulin.
社団法人日本生物工学会の論文
1952-08-15