非醗酵性糖に關する研究(第6報) : glucoseからisomaltoseの生成に就て

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Studying the production of isomaltose by action of fungal enzymes on glucose, the following results were obtained.Filtrates from fungal culture (Asp. niger sp.) were added as fungal enzymes to the buffered glucose solutions and the reaction mixtures were incubated for 72 hrs. at 55C. The synthesized sugars were examined on a paper chromatography. Isoamltose, sakebiose and kojibiose were synthesized from glucose. (Isomaltose, yield is about 3%. sakebiose and kojibiose, total yield is 0.79%). Isoamltose was isolated from the enzymatic reaction mixtures, using a modification of the method described by WHISTLER and DURSO. The obtained isomaltose was acetylated. Isomaltose octaacetate M.P. 143〜145℃, isomaltose phenylosazone M.P. 206〜208℃.
社団法人日本生物工学会の論文
1953-09-15