Systemic Release of Mucosal Mast-cell Protease in Primed Brown Norway Rats after Feeding with β-Lactoglobulin

概要

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The plasma level of mucosal mast-cell protease was examined to find whether such measurements could be an indicator of allergic response to β-lactoglobulin (β-LG) challenged orally by rats. Brown Norway rats, which had been raised on a bovine milk-free diet, were systemically sensitized on day 0 with a low dose of β-LG, and then by an oral administration of β-LG for 3h on day 14. The oral challenge with β-LG in saline, when compared to saline alone, resulted in a systemic elevation of rat mast-cell protease II (RMCPII), one of the specific markers for gut mucosal mast-cell secretion. The challenge with β-LG in a fat emulsion further increased the level of plasma RMCPII. This manipulation, however, was not successful for detecting any significant difference in mucosal leucotriene C_4, another allergic mediator. An oral challenge with polymerized β-LG did not induce any elevation of the protease, but resulted in a lower plasma level of β-LG-specific IgG. This animal model is thus relevant to investigate the events regulating the mucosal hypersensitivity and humoral immunity to food proteins.
社団法人日本農芸化学会の論文
1995-05-23