Optimization of Bovine Cathepsin C Production by Cultivation of Recombinant Methylotrophic Yeast Candida boidinii
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概要
- 論文の詳細を見る
Bovine cathepsin C (CTC) production from a recombinant methylotrophic yeast Candida boidinii CT714 was investigated, in which CTC gene was driven by formate dehydrogenase promoter and strongly induced in the presence of methanol. Two step fermentation using DYS medium containing 1.0% (w/v) glucose for cell growth and MYS medium containing 2.5% (v/v) methanol for inducible production of CTC was suitable for efficient production. Methanol concentration within 1.5 to 3.5% (v/v) was preferable for CTC induction. After 24 h of cell growth and 72 h of induction, CTC production reached approximately 12 U/I (this corresponded to 2.15 U/g-dry cells). When vitamins were enriched in growth medium, CTC productivity was improved more than 3 times higher, and it was found that the effective vitamins were biotin, p-aminobenzoic acid and thioctic acid.
- 社団法人 化学工学会の論文
著者
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Kano Kenjiro
Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.
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KIKUTA HIROKAZU
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Komeda T
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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Kikuta Hirokazu
Department Of Biotechnology School Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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