Degradation of Nucleic Acids with Ozone. III. Mode of Ozone-Degradation of Mouse Proline Transfer Ribonucleic Acid (tRNA) and Isoleucine tRNA

スポンサーリンク

概要

• 論文の詳細を見る

• The degradation of mouse [^<32>P]proline transfer ribonucleic acid (tRNA) and [^<32>P]isoleucine tRNA with ozone (concentration in inlet gas, 0.1 mg/l) was examined in aqueous solution (reaction time, 0-32 min) and sequence analysis of the products was performed by digestion with ribonuclease A or T_1 according to Sanger's method. The guanine moieties in the nucleobases were preferentially attacked by ozone and the cleavage of polynucleotidic linkages did not occur during the ozonization. Proline tRNA was more degradable than isoleucine tRNA, probably as a result of differences in the locations of the guanine residues in their higher-order structures. The consecutive guanine residues in the anticodon and dihydrouracil (D) regions of proline tRNA were most susceptible to degradation with ozone. In the case of isoleucine tRNA, which does not have the conseutive guanine residues in the anticodon region, those in the D-loop, small (S) loop and the common stem were degraded. Therefore ozone seems to start reacting with the guanine moieties located in the most exposed regions of the higher-order structure of tRNA.

• 公益社団法人日本薬学会の論文
• 1983-10-25

著者

• 上田 亨

  Faculty of Pharmaceutical Science, Hokkaido University

• 上田 亨

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 原田 文夫

  Virology Division, National Cancer Center Research Institute

• 三浦 一伸

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 神力 就子

  北海道工開試

• 神力 就子

  工技院北海道工業開発試験所

• 石崎 紘三

  Government Industrial Development Laboratory, Hokkaido

• 神力 就子

  Government Industrial Development Laboratory, Hokkaido

• 上田 亨

  北海道大学薬学部

• 原田 文夫

  Virology Division National Cancer Center Research Institute

• 三浦 一伸

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 原田 文夫

  National Cancer Center Research Institute

関連論文

• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• PHOSPHOLIPASE D-CATALYZED TRANS-ALKYLPHOSPHORYLATION : A FACILE ONE-STEP SYNTHESIS OF NUCLEOSIDE 5'-ALKYLPHOSPHATES
• Synthesis of Bredinin from 1-β-D-Ribofuranosyl-5-aminoimidazole-4-carboxamide by a Photo-Reaction
• Synthesis of 6,2'-Methano-cyclouridine, a Uridine Fixed in High-Anti Conformation (Nucleosides and Nucleotides. LX)
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Nucleosides and Nucleotides. CXIX. Inhibition of DNA-Cytosine Methylase Hha I by a Self-Complementary Oligonucleotide Containing 5-Fluorocytosine
• 水-水素同位体交換反応を利用する重水濃度の迅速定量法
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Pyrimidine S- and N-Cyclonucleosides. Synthesis of 6,2'(and 5')-S- and N-Cyclouridines (Nucleosides and Nucleotides. XXXII)
• Nucleosides and Nucleotides. IX. Synthesis of Sulfur-bridged Cyclonucleosides : (S)-2,2'-, 2,3'-, and 2,5'-Cyclo-2-thiouridines
• Synthesis of Sulfur-Bridged Uracil Anhydronucleosides
• Nucleotide Sequence of 5S Ribosomal Ribonucleic Acid from a Sulfate-Reducing Bacterium, Desulfovibrio vulgaris
• Modification of Adenine Residues of Mouse 5S Ribosomal Ribonucleic Acid with Monoperphthalic Acid ; the Secondary Structure of 5S Ribosomal Ribonucleic Acid (Nucleosides and Nucleotides. LVI)
• Blockwise Mechanical Synthesis of Oligonucleotides by the Phosphoramidite Method(Organic,Chemical)
• Deoxyribonucleic Acids and Related Compounds. XXII. : Synthesis of Genes for Human Nerve Growth Factor and Its Fused Protein
• Reaction of 5-Bromouridine Derivatives with Dimethyl Malonate Carbanion. A Novel Entry to the Synthesis of Uridine-5-acetic Acids
• Synthesis of 2'-Deoxy-6,2'-methano-cyclouridine (Nucleosides and Nucleotides. LIX)
• Nucleosides and Nucleotides. LIV. Synthesis of 6,6'-Cyclo-5', 6'-dideoxyhexofuranosyluracils via Radical Cyclization of a 6-Cyanouridine
• Introduction of Carbon Substituents into Pyrimidine and Purine Nucleosides by Sulfur Extrusion (Nucleosides and Nucleotides. XXX)
• Synthesis of 6,5'-S- and 6,5'-N-Cyclouridines (Nucleosides and Nucleotides. XXII)
• Synthesis of 6-Cyano- and 5-Cyano-uridines and Their Derivatives (Nucleosides and Nucleotides. XXI)
• Synthesis of 6-Cyanocytidine and Its Derivatives (Nucleosides and Nucleotides. XX
• Nucleosides and Nucleotides. XIV. Synthesis of 6-Alkylthiouridines and Uridine-6-sulfonic Acid
• Synthesis of Orotidine from Uridine
• THE DESIGN, SYNTHESIS AND ANTILEUKEMIC ACTIVITY OF 5-ALKYNYL-1-β-D-RIBOFURANOSYLIMIDAZOLE-4-CARBOXAMIDES
• Substrate Specificity of a Thymidine Phosphorylase in Human Liver Tumor
• 44.オゾン水溶液中におけるBacillus subtilis胞子の不活性化(第66回 日本医科器械学会大会 一般演題講演集)
• (44) オゾン水溶液中におけるBacillus subtilisi胞子の不活性化
• Degradation of Nucleic Acids with Ozone. VI. Labilization of the Double-Helical Structure of Calf Thymus Deoxyribonucleic Acid
• Degradation of Nucleic Acids with Ozone. V. Mechanism of Action of Ozone on Deoxyribonucleoside 5'-Monophosphates
• Alternative Synthesis of 2'-Deoxy-6,2'-methano-pyrimidine Nucleosides : Nucleosides and Nucleotides. XC
• Synthesis of 6,6'-Cyclo-5', 6'-dideoxy-1-(β-D-allofuranosyl)cytosine and Related Nucleosides : Nucleosides and Nucleotides.LXXXVIII)
• Synthesis of 6,3'-Methanocytidine, 6,3'-Methanouridine, and Their 2'-Deoxyribonucleosides (Nucleosides and Nucleotides. LXXVII)
• Preparation and Characterization of Monoclonal Antibodies Specific for Ribonuclese Inhibitor from Rat Liver
• Overproduction of Cellular and Activated Ha-ras Proteins by Mutating a Synthetic Gene(Biological,Chemical)
• Purification of Chicken Liver Ribonucleases by Affinity Chromatography with UMP-Sepharose (Nucleosides and Nucleotides. LII)
• Alkyl Addition Reaction of Pyrimidine 2'-Ketonucleosides : Synthesis of 2'-Branched-Chain Sugar Pyrimidine Nucleosides : Nucleosides and Nucleotides. LXXXI^1
• ガスクロマトグラフィーによる重水素濃度の高感度迅速定量法
• ステロイドの分析化学的研究(第45報)Androst-4-ene-3,6,17-trioneのポーラログラフ的挙動
• ステロイドの分析化学的研究(第39報) : ケトステロイドメチルオキシム誘導体のポーラログラフィー
• ステロイドの分析化学的研究(第38報) : 不飽和ケトステロイドのポーラログラフィー
• フェニルスルホン類のポーラログラフィー
• チアクロモン1,1-ジオキシド類のボーラログラフィー
• The Sulfone Stretching Frequencies of Some Phenylsulfonyl Derivatives.
• 半数をくじ引きに : 入学試験をどう位置づけるか(入試制度と薬学教育)
• Molecular Conformations of Carbon-Bridged Cyclodeoxyadenosines in the Solid State and in Solution.2'-Deoxy-8,2'-methanoadenosine and 2'-Deoxy-8,2'-ethanoadenosine
• Nucleosides and Nucleotides. XLIII. : On the Stereoselectivity of Alkyl Addition Reaction of Pyrimidine 2'-Ketonucleosides
• Crystal and Molecular Structures of Carbon-Bridged Pyrimidine Cyclonucleosides. Substrate Analogs of Ribonuclease A
• A NEW METHOD FOR SYNTHESIZING THE ANTINEOPLASTIC NUCLEOSIDES 1-(2-AZIDO-2-DEOXY-β-D-ARABINOFURANOSYL)CYTOSINE (CYTARAZID) AND 1-(2-AMINO-2-DEOXY-β-D-ARABINOFURANOSYL)CYTOSINE (CYTARAMIN) FROM URIDINE
• Synthesis and Optical Properties of 2'-Deoxy-8,2'-methanoguanosine
• 欲のぶつけ合いの中から(研究者の能力を高めるために実行している教育)
• A Facile Enzymatic Synthesis of 5'-(3-sn-Phosphatidyl)nucleosides and Their Antileukemic Activities
• 57(PB1-6) 2'-分枝糖ピリミジンヌクレオシドの合成(ポスター発表の部)
• Synthesis of 6,5'-Cyclo-5'-deoxy-5'(R and S)-(2-hydroxyethyl)-uridmes (Nucleosides and Nucleotides. LXXIII)(Organic,Chemical)
• Synthesis of 6,5'-Cyclo-2',5'-dideoxypyrimidine Nucleosides : Nucleosides and Nucleotides. LXXII(Organic,Chemical)
• CONVERSION OF GUANOSINE INTO 2-AMINOMETHYLINOSINE (2-HOMOGUANOSINE)
• The Reaction of 6-Phenylthiouridine with Sulfur Nucleophiles : A Simple and Regiospecific Preparation of 6-Alkylthiouridines and 6-Alkylthiouridylic Acids
• Potential Antimetabolites. IV. Synthesis of 2,6-Bis-alkylthiopurine Ribosides and their Selective Substitution by Nucleophilic Reagents.
• Ribozyme--酵素としてのRNA (1987年の化学-5-)
• ras遺伝子産物の活性と構造 (1986年の化学-4-)
• 遺伝子の人工合成とその発現 (1985年の化学)
• Synthesis of Guanosine 5'-Diphosphate.
• Debenzylation Reaction of Benzylphosphoric Acid Esters
• Nucleosides and Nucleotides. XXXXV. Facile Deoxygenation of Neplanocin A and Nucleosides by the Use of Tri-n-butyltin Hydride
• Synthesis of 2'-Substituted Derivatives of Neplanocin A (Nucleosides and Nucleotides. XLIV)
• Synthesis of 2'(R)-Substituted Neplanocin A's (Nucleosides and Nucleotides. XXXVII)
• Synthesis and Optical Properties of 2,3-Dideoxy-D-erythro-hex-2-enopyranosyl Nucleosides (Nucleosides and Nucleotides. LXII)
• Degradation of Nucleic Acids with Ozone. IV. Specific Internucleotidic Bond-Cleavage of Ozone-Treated Transfer Ribonucleic Acids with Aniline-acetate
• Degradation of Nucleic Acids with Ozone. III. Mode of Ozone-Degradation of Mouse Proline Transfer Ribonucleic Acid (tRNA) and Isoleucine tRNA
• Degradation of Nucleic Acids with Ozone. I. Degradation of Nucleobases, Ribonucleosides and Ribonucleoside-5'-monophosphates
• Preparation of ^S-Labeled Ribonucleic Acid and Deoxyribonucleic Acid and Their Use for the Assay of Nucleolytic Enzymes (Nucleosides and Nucleotides. XXXIX)
• Modification of Cytosine Moieties of Nucleic Acids with Hydrogen Sulfide (Nucleosides and Nucleotides. XXXIV)
• Synthesis of 6-Thioguanine and 2,6-Diaminopurine Nucleosides and Nucleotides from Adenine Counterparts via a Facile Rearrangement in the Base Portion (Nucleosides and Nucleotides. XIX)
• Nucleosides and Nucleotides. XIII. Synthesis of Thiopurine Nucleosides from Adenosine and Guanosine Derivatives by the Sulfhydrolysis
• Chemical Conversion of Adenosine to Guanosine (Nucleosides and Nucleotides. XI)
• Nucleosides and Nucleotides. X. Synthesis of 4-Thiouracil Nucleosides and Nucleotides by the Solvolysis of Cytidine and Its Phosphates with Hydrogen Sulfide
• Nucleosides and Nucleotides. VIII. Formation of Spongocytidylyladenosine in the Synthesis of Cytidylyladenosine from Cytidine 2', 3'-Cyclic Phosphate and Adenosine
• Reaction of 6-Methylsulfonylpurine Riboside with Carbon Nucleophiles and the Synthesis of 6-Alkylpurine Nucleosides (Nucleosides and Nucleotides. XXIX)
• Synthesis of 2,4-Dithiouridine and 2-Thiocytidine
• A Convenient Synthesis of Ribonucleoside 2', 3', -Cyclic Phosphates from Ribonucleosides and Ribonucleotides
• Interactions between Synthetic Nucleotide Analogs and Snake Venom 5'-Nucleotidase
• A New and Convenient Synthesis of 1-O-Acetyl-2,3,5-tri-O-benzoyl-β-D-ribofuranose from Guanosine ; Some Observations on Solvolysis of 2', 3', 5'-Tri-O-benzoylguanosine
• Chemical Synthesis of Guanosine Diphosphate Mannose (GDPM)
• Synthesis of 8,2'-Methanoguanosine and 9-(α-D-Arabinofuranosyl)-8,2'-methanoguanine (Nucleosides and Nucleotides. LXVII.)
• Synthesis of 8,6'-Cyclo-6'-deoxyhexofuranosyladenines : Adenosines Fixed in an Anti-conformation (Nucleosides and Nucleotides. LXVI)
• Synthesis of 8,2'-Methano- and 8,2'-Ethanoadenosines(Nucleosides and Nucleotides. LXV)
• A FACILE SYNTHESIS OF 6,3'-METHANO-URIDINE AND-CYTIDINE FROM A 3-KETOSUGAR : NUCLEOSIDES AND NUCLEOTIDES LXVIII
• Synthesis of 2'-Deoxy-8,2'-ethanoadenosine and 3'-Deoxy-8,3'-ethanoadenosine : Nucleosides and Nucleotides. LXIV
• Synthesis of 8-Alkyladenosines, 8,2'-Anhydro-8-hydroxymethyl-9-(β-D-arabinofuranosyl) adenine and Related Compounds (Nucleosides and Nucleotides. LVIII)
• Synthesis and Optical Properties of 2,5'-O-Cycloimidazole Nucleosides and Related Compounds (Nucleosides and Nucleotides. XXV)
• Nucleosides and Nucleotides. XII. Synthesis and Properties of 2'-Deoxy-2'-mercaptouridine and Its Derivatives
• Synthesis of 2-Thiouridine and 6-Methyl-3-(β-D-ribofuranosyl)-2-thiouracil
• Synthesis of 5'-Hydroxyalkyl-5'-deoxy-8,5'(R and S)-cycloadenosines (Nucleosides and Nucleotides. XXXI)
• Synthesis of 2- and 8-Cyanoadenosines and Their Derivatives : Nucleosides and Nucleotides. XXVII
• Optical Properties of Thiopyrimidine Nucleosides
• Nucleosides and Nucleotides. VII. Synthesis of 2-Thiocytidine by the Extended Hilbert-Johnson Procedure
• Nucleosides and Nucleotides. V. Identification and Substitution Reaction of 1-Methyl-2,4-dialkoxypyrimidinium Salts : Model Studies on Hilbert-Johnson Pyrimidine Nucleoside Synthesis
• Coenzyme Analogs. XI. Synthesis of N-Methyl-and N, N-Dimethylcytidine 5'-Phosphate.

もっと見る 閉じる

スポンサーリンク