Purification of Chicken Liver Ribonucleases by Affinity Chromatography with UMP-Sepharose (Nucleosides and Nucleotides. LII)

スポンサーリンク

概要

• 論文の詳細を見る

• A procedure for the preparation of chicken liver ribonucleases (RNases) is described, involving affinity chromatography on Sepharose coupled with 5'-amino-5'-deoxyuridine 2'(3')-phosphate. Two kinds of RNases having acidic pH optima were obtained. One RNase exhibited preferential nucleolytic activity toward poly C rather than poly U and the other was specific for poly U. The former RNase may be identical to that reported by Levy et al. and the latter resembles acid RNase detected in several mammalian tissues.

• 社団法人日本薬学会の論文
• 1984-10-25

著者

• 上田 亨

  Faculty of Pharmaceutical Science, Hokkaido University

• 上田 亨

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 三浦 一伸

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 井上 泰彦

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 井上 泰彦

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 橋本 由佳理

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 井上 淳子

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 上田 亨

  北海道大学薬学部

• 井上 淳子

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 三浦 一伸

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 橋本 由佳理

  Faculty Of Pharmaceutical Sciences Hokkaido University

関連論文

• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• PHOSPHOLIPASE D-CATALYZED TRANS-ALKYLPHOSPHORYLATION : A FACILE ONE-STEP SYNTHESIS OF NUCLEOSIDE 5'-ALKYLPHOSPHATES
• Synthesis of Bredinin from 1-β-D-Ribofuranosyl-5-aminoimidazole-4-carboxamide by a Photo-Reaction
• Synthesis of 6,2'-Methano-cyclouridine, a Uridine Fixed in High-Anti Conformation (Nucleosides and Nucleotides. LX)
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Nucleosides and Nucleotides. CXIX. Inhibition of DNA-Cytosine Methylase Hha I by a Self-Complementary Oligonucleotide Containing 5-Fluorocytosine
• Studies on Antitumor Agents. IX. : Synthesis of 3'-O-Benzyl-2'-deoxy-5-trifluoromethyluridine
• Pyrimidine S- and N-Cyclonucleosides. Synthesis of 6,2'(and 5')-S- and N-Cyclouridines (Nucleosides and Nucleotides. XXXII)
• Nucleosides and Nucleotides. IX. Synthesis of Sulfur-bridged Cyclonucleosides : (S)-2,2'-, 2,3'-, and 2,5'-Cyclo-2-thiouridines
• Synthesis of Sulfur-Bridged Uracil Anhydronucleosides
• Nucleotide Sequence of 5S Ribosomal Ribonucleic Acid from a Sulfate-Reducing Bacterium, Desulfovibrio vulgaris
• Modification of Adenine Residues of Mouse 5S Ribosomal Ribonucleic Acid with Monoperphthalic Acid ; the Secondary Structure of 5S Ribosomal Ribonucleic Acid (Nucleosides and Nucleotides. LVI)
• Blockwise Mechanical Synthesis of Oligonucleotides by the Phosphoramidite Method(Organic,Chemical)
• Deoxyribonucleic Acids and Related Compounds. XXII. : Synthesis of Genes for Human Nerve Growth Factor and Its Fused Protein
• Reaction of 5-Bromouridine Derivatives with Dimethyl Malonate Carbanion. A Novel Entry to the Synthesis of Uridine-5-acetic Acids
• Synthesis of 2'-Deoxy-6,2'-methano-cyclouridine (Nucleosides and Nucleotides. LIX)
• Nucleosides and Nucleotides. LIV. Synthesis of 6,6'-Cyclo-5', 6'-dideoxyhexofuranosyluracils via Radical Cyclization of a 6-Cyanouridine
• Introduction of Carbon Substituents into Pyrimidine and Purine Nucleosides by Sulfur Extrusion (Nucleosides and Nucleotides. XXX)
• Synthesis of 6,5'-S- and 6,5'-N-Cyclouridines (Nucleosides and Nucleotides. XXII)
• Synthesis of 6-Cyano- and 5-Cyano-uridines and Their Derivatives (Nucleosides and Nucleotides. XXI)
• Synthesis of 6-Cyanocytidine and Its Derivatives (Nucleosides and Nucleotides. XX
• Nucleosides and Nucleotides. XIV. Synthesis of 6-Alkylthiouridines and Uridine-6-sulfonic Acid
• Synthesis of Orotidine from Uridine
• THE DESIGN, SYNTHESIS AND ANTILEUKEMIC ACTIVITY OF 5-ALKYNYL-1-β-D-RIBOFURANOSYLIMIDAZOLE-4-CARBOXAMIDES
• Substrate Specificity of a Thymidine Phosphorylase in Human Liver Tumor
• Degradation of Nucleic Acids with Ozone. VI. Labilization of the Double-Helical Structure of Calf Thymus Deoxyribonucleic Acid
• A Convenient Synthesis of Diribonucleoside Monophosphates by the Use of Unblocked Nucleosides
• Synthetic Studies of Potential Antimetabolites. XI. Syntheses of β-D-Pentofuranosyl-5-methylcytosines
• Alternative Synthesis of 2'-Deoxy-6,2'-methano-pyrimidine Nucleosides : Nucleosides and Nucleotides. XC
• Synthesis of 6,6'-Cyclo-5', 6'-dideoxy-1-(β-D-allofuranosyl)cytosine and Related Nucleosides : Nucleosides and Nucleotides.LXXXVIII)
• Synthesis of 6,3'-Methanocytidine, 6,3'-Methanouridine, and Their 2'-Deoxyribonucleosides (Nucleosides and Nucleotides. LXXVII)
• Preparation and Characterization of Monoclonal Antibodies Specific for Ribonuclese Inhibitor from Rat Liver
• Overproduction of Cellular and Activated Ha-ras Proteins by Mutating a Synthetic Gene(Biological,Chemical)
• Purification of Chicken Liver Ribonucleases by Affinity Chromatography with UMP-Sepharose (Nucleosides and Nucleotides. LII)
• Alkyl Addition Reaction of Pyrimidine 2'-Ketonucleosides : Synthesis of 2'-Branched-Chain Sugar Pyrimidine Nucleosides : Nucleosides and Nucleotides. LXXXI^1
• Stereochemistry of Microbial Hydrogenation of (-)-α-Santonin to (+)-1,2-Dihydro-α-santonin by Streptomyces cinereocrocatus NRRL 3443
• 半数をくじ引きに : 入学試験をどう位置づけるか(入試制度と薬学教育)
• Molecular Conformations of Carbon-Bridged Cyclodeoxyadenosines in the Solid State and in Solution.2'-Deoxy-8,2'-methanoadenosine and 2'-Deoxy-8,2'-ethanoadenosine
• Nucleosides and Nucleotides. XLIII. : On the Stereoselectivity of Alkyl Addition Reaction of Pyrimidine 2'-Ketonucleosides
• Crystal and Molecular Structures of Carbon-Bridged Pyrimidine Cyclonucleosides. Substrate Analogs of Ribonuclease A
• A NEW METHOD FOR SYNTHESIZING THE ANTINEOPLASTIC NUCLEOSIDES 1-(2-AZIDO-2-DEOXY-β-D-ARABINOFURANOSYL)CYTOSINE (CYTARAZID) AND 1-(2-AMINO-2-DEOXY-β-D-ARABINOFURANOSYL)CYTOSINE (CYTARAMIN) FROM URIDINE
• Synthesis and Optical Properties of 2'-Deoxy-8,2'-methanoguanosine
• 欲のぶつけ合いの中から(研究者の能力を高めるために実行している教育)
• A Facile Enzymatic Synthesis of 5'-(3-sn-Phosphatidyl)nucleosides and Their Antileukemic Activities
• 57(PB1-6) 2'-分枝糖ピリミジンヌクレオシドの合成(ポスター発表の部)
• Synthesis of 6,5'-Cyclo-5'-deoxy-5'(R and S)-(2-hydroxyethyl)-uridmes (Nucleosides and Nucleotides. LXXIII)(Organic,Chemical)
• Synthesis of 6,5'-Cyclo-2',5'-dideoxypyrimidine Nucleosides : Nucleosides and Nucleotides. LXXII(Organic,Chemical)
• CONVERSION OF GUANOSINE INTO 2-AMINOMETHYLINOSINE (2-HOMOGUANOSINE)
• The Reaction of 6-Phenylthiouridine with Sulfur Nucleophiles : A Simple and Regiospecific Preparation of 6-Alkylthiouridines and 6-Alkylthiouridylic Acids
• 社会薬学の必要性
• Potential Antimetabolites. IV. Synthesis of 2,6-Bis-alkylthiopurine Ribosides and their Selective Substitution by Nucleophilic Reagents.
• Ribozyme--酵素としてのRNA (1987年の化学-5-)
• ras遺伝子産物の活性と構造 (1986年の化学-4-)
• 遺伝子の人工合成とその発現 (1985年の化学)
• Synthesis of Guanosine 5'-Diphosphate.
• Debenzylation Reaction of Benzylphosphoric Acid Esters
• Nucleosides and Nucleotides. XXXXV. Facile Deoxygenation of Neplanocin A and Nucleosides by the Use of Tri-n-butyltin Hydride
• Synthesis of 2'-Substituted Derivatives of Neplanocin A (Nucleosides and Nucleotides. XLIV)
• Synthesis of 2'(R)-Substituted Neplanocin A's (Nucleosides and Nucleotides. XXXVII)
• Synthesis and Optical Properties of 2,3-Dideoxy-D-erythro-hex-2-enopyranosyl Nucleosides (Nucleosides and Nucleotides. LXII)
• 有機化学と薬理学の橋渡しとして(薬品化学で何を教えるか)
• ミキサーは研究の発表・交流の夜の部として位置づけ, 懇親会は学会運営を語る場(年会懇親会のあり方)
• オクテット
• Degradation of Nucleic Acids with Ozone. IV. Specific Internucleotidic Bond-Cleavage of Ozone-Treated Transfer Ribonucleic Acids with Aniline-acetate
• Degradation of Nucleic Acids with Ozone. III. Mode of Ozone-Degradation of Mouse Proline Transfer Ribonucleic Acid (tRNA) and Isoleucine tRNA
• Degradation of Nucleic Acids with Ozone. I. Degradation of Nucleobases, Ribonucleosides and Ribonucleoside-5'-monophosphates
• Preparation of ^S-Labeled Ribonucleic Acid and Deoxyribonucleic Acid and Their Use for the Assay of Nucleolytic Enzymes (Nucleosides and Nucleotides. XXXIX)
• Modification of Cytosine Moieties of Nucleic Acids with Hydrogen Sulfide (Nucleosides and Nucleotides. XXXIV)
• Synthesis of 6-Thioguanine and 2,6-Diaminopurine Nucleosides and Nucleotides from Adenine Counterparts via a Facile Rearrangement in the Base Portion (Nucleosides and Nucleotides. XIX)
• Nucleosides and Nucleotides. XIII. Synthesis of Thiopurine Nucleosides from Adenosine and Guanosine Derivatives by the Sulfhydrolysis
• Chemical Conversion of Adenosine to Guanosine (Nucleosides and Nucleotides. XI)
• Nucleosides and Nucleotides. X. Synthesis of 4-Thiouracil Nucleosides and Nucleotides by the Solvolysis of Cytidine and Its Phosphates with Hydrogen Sulfide
• Nucleosides and Nucleotides. VIII. Formation of Spongocytidylyladenosine in the Synthesis of Cytidylyladenosine from Cytidine 2', 3'-Cyclic Phosphate and Adenosine
• Reaction of 6-Methylsulfonylpurine Riboside with Carbon Nucleophiles and the Synthesis of 6-Alkylpurine Nucleosides (Nucleosides and Nucleotides. XXIX)
• 核酸の機能・構造研究と化学修飾
• 合成遺伝子による蛋白質の生産と改変
• Synthesis of 2,4-Dithiouridine and 2-Thiocytidine
• A Convenient Synthesis of Ribonucleoside 2', 3', -Cyclic Phosphates from Ribonucleosides and Ribonucleotides
• Interactions between Synthetic Nucleotide Analogs and Snake Venom 5'-Nucleotidase
• A New and Convenient Synthesis of 1-O-Acetyl-2,3,5-tri-O-benzoyl-β-D-ribofuranose from Guanosine ; Some Observations on Solvolysis of 2', 3', 5'-Tri-O-benzoylguanosine
• Chemical Synthesis of Guanosine Diphosphate Mannose (GDPM)
• Synthesis of 8,2'-Methanoguanosine and 9-(α-D-Arabinofuranosyl)-8,2'-methanoguanine (Nucleosides and Nucleotides. LXVII.)
• Synthesis of 8,6'-Cyclo-6'-deoxyhexofuranosyladenines : Adenosines Fixed in an Anti-conformation (Nucleosides and Nucleotides. LXVI)
• Synthesis of 8,2'-Methano- and 8,2'-Ethanoadenosines(Nucleosides and Nucleotides. LXV)
• A FACILE SYNTHESIS OF 6,3'-METHANO-URIDINE AND-CYTIDINE FROM A 3-KETOSUGAR : NUCLEOSIDES AND NUCLEOTIDES LXVIII
• Synthesis of 2'-Deoxy-8,2'-ethanoadenosine and 3'-Deoxy-8,3'-ethanoadenosine : Nucleosides and Nucleotides. LXIV
• Synthesis of 8-Alkyladenosines, 8,2'-Anhydro-8-hydroxymethyl-9-(β-D-arabinofuranosyl) adenine and Related Compounds (Nucleosides and Nucleotides. LVIII)
• Synthesis and Optical Properties of 2,5'-O-Cycloimidazole Nucleosides and Related Compounds (Nucleosides and Nucleotides. XXV)
• Nucleosides and Nucleotides. XII. Synthesis and Properties of 2'-Deoxy-2'-mercaptouridine and Its Derivatives
• シクロヌクレオシドの化学
• Synthesis of 2-Thiouridine and 6-Methyl-3-(β-D-ribofuranosyl)-2-thiouracil
• 5′-デオキシ-8,5′-シクログアノシン=2′,3′-環状リン酸エステルおよび3′-リン酸エステルの合成とリボヌクレア-ゼT1との相互作用 (生物活性物質)
• Synthesis of 5'-Hydroxyalkyl-5'-deoxy-8,5'(R and S)-cycloadenosines (Nucleosides and Nucleotides. XXXI)
• Synthesis of 2- and 8-Cyanoadenosines and Their Derivatives : Nucleosides and Nucleotides. XXVII
• Optical Properties of Thiopyrimidine Nucleosides
• Nucleosides and Nucleotides. VII. Synthesis of 2-Thiocytidine by the Extended Hilbert-Johnson Procedure
• Nucleosides and Nucleotides. V. Identification and Substitution Reaction of 1-Methyl-2,4-dialkoxypyrimidinium Salts : Model Studies on Hilbert-Johnson Pyrimidine Nucleoside Synthesis
• Coenzyme Analogs. XI. Synthesis of N-Methyl-and N, N-Dimethylcytidine 5'-Phosphate.

もっと見る 閉じる

スポンサーリンク