Preparation of ^<35>S-Labeled Ribonucleic Acid and Deoxyribonucleic Acid and Their Use for the Assay of Nucleolytic Enzymes (Nucleosides and Nucleotides. XXXIX)

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概要

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• Radioactive sulfur was introduced into ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) molecules by chemical means. The thio group in 4-thiouracil residues, which were introduced into RNA and DNA by sulfhydrolysis, was exchanged with radioactive Na_2S via a 4-thiocyanatouracil intermediate. Specific activity of the ^<35>S-labeled RNA and DNA was around 10^5 cpm/ODU_260. The labeled RNA and DNA were successfully applied as substrates for the assay of nucleolytic enzymes or ribonuclease inhibitor. The sensitivity of the assay was 100 times better than that of the current method using non-labeled nucleic acids as the substrate.

• 公益社団法人日本薬学会の論文
• 1982-03-25

著者

• 上田 亨

  Faculty of Pharmaceutical Science, Hokkaido University

• 高木 久美子

  Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University

• 三浦 一伸

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 佐藤 祥子

  Government Industrial Development Laboratory Hokkaido

• 上田 亨

  北海道大学薬学部

• 三浦 一伸

  Faculty Of Pharmaceutical Sciences Hokkaido University

• 佐藤 祥子

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 遠山 純一

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 高木 久美子

  Faculty Of Pharmaceutical Sciences Toyama Medical & Pharmaceutical University

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