合成遺伝子による蛋白質の生産と改変
スポンサーリンク
概要
- 論文の詳細を見る
In order to clarify the structure-activity relationship of c-Ha-<I>ras</I> proteins (p 21 s) genes encoding p 21 (Val-12), p 21 (Leu-61) and p 21 (Arg-61) were synthesized by joining oligonucleotides with T 4 DNA ligase and expressed in <I>E. coli</I> under the regulation of <I>E. coli</I> tryptophan promoter. In addition, the gene for normal p 21 was constructed by cassette mutagenesis using restriction sites, <I>Cla</I>I-<I>Bss</I>H II. The guanosine diphosphate (triphosphate) binding properties and guanosine triphosphatase (GTP<SUB>ase</SUB>) activities of these p 21 s were examined. It was found that the guanine nucleotide binding abilities of all p 21 s produced in this study were relatively same but GTP<SUB>ase</SUB> activity of activated p 21 s were significantly reduced.<BR>Furthermore, the gene encoding novel p 21 in which the guanine nucleotide binding sites was modified was synthesized by ligation of oligonucleotides and expressed in <I>E.coli</I>. Its biochemical activities are also discussed.
- 社団法人 有機合成化学協会の論文
著者
-
紙谷 浩之
北海道大学大学院薬学研究院
-
大塚 栄子
北海道大学大学院薬学研究科
-
三浦 一伸
Faculty Of Pharmaceutical Sciences Hokkaido University
-
三浦 一伸
(株) 関西新技術研究所 大阪研究所
-
沢田石 一之
(株) 関西新技術研究所 大阪研究所
-
大塚 栄子
北海道大学薬学部製薬化学科
関連論文
- P-116 出芽酵母における脱アミノ化ヌクレオチド損傷浄化機構とゲノム安定性への寄与(ポスターセッション)
- 多機能性エンベロープ型人工遺伝子デリバリーシステムの創製
- 多機能性エンベロープ型ナノ構造体による人工遺伝子デリバリーシステムの創製 : 第7回日本DDS学会永井賞受賞によせて
- 多機能性エンベロープ型ナノ構造体の開発 : Programmed Packaging の提唱
- 核酸の化学合成(遺伝子に化学のメスを入れる 1)
- 真核生物の複製フォーク複合体の分子集合
- Nucleotide Sequence of 5S Ribosomal Ribonucleic Acid from a Sulfate-Reducing Bacterium, Desulfovibrio vulgaris
- Modification of Adenine Residues of Mouse 5S Ribosomal Ribonucleic Acid with Monoperphthalic Acid ; the Secondary Structure of 5S Ribosomal Ribonucleic Acid (Nucleosides and Nucleotides. LVI)
- 酸素ラジカル及び一酸化窒素(NO)によって生ずるDNA損傷がNIH3T3細胞中で誘発する点変異
- Blockwise Mechanical Synthesis of Oligonucleotides by the Phosphoramidite Method(Organic,Chemical)