Isolation of Dog, Rat and Hog Pancreatic Kallikreins by Preparative Disc Electrophoresis and Their Properties
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概要
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Dog, rat and hog pancreatic kallikreins were efficiently purified to homogeneous forms by combining preparative disc electrophoresis with acetone fractionation and Sephadex gel filtration. The recoveries of these kallikreins through the whole purification procedures were in the range of 20-45% in vasodilator and N^a-benzoyl-L-arginine ethyl ester (BAEE) assays. The purified kallikreins appeared to be homogeneous in analytical disc electrophoresis ; however, isoelectric focusing made it clear that they consisted of several kallikrein multiple forms with slightly different isoelectric points (pI's). The pI's of these kallikreins'multiple forms wer 4.2-4.5 (dog), 4.1 (rat), and 3.6-4.2 (hog). Dog pancreatic kallikrein was not inhibited by Trasylol, but were inhibited considerably by two kallikrein inhibitors from potatoes. Although rat kallikrein has been reported not to be inhibited by Trasylol, our purified one was strongly inhibited. Trypsin inhibitors from soybean etc. hardly inhibited both kallikreins.
- 社団法人日本薬学会の論文
- 1975-05-25
著者
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森 弘
Chemical Research Department Teikoku Hormone Mfg. Co. Ltd.
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法島 義男
Faculty of Pharmaceutical Sciences Science University of Tokyo
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森脇 千秋
Faculty of Pharmaceutical Sciences Science University of Tokyo
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森 弘
Faculty of Pharmaceutical Sciences Science University of Tokyo
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森脇 千秋
Laboratory Of Physiological Chemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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法島 義男
Faculty Of Pharmaceutical Sciences Science University Of Tokyo:(present Address)department Of Immuno
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森脇 千秋
Laboratory of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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