Purification and Characterization of Dog Urinary Kallikrein

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概要

• 論文の詳細を見る

• Two forms of N-α-tosyl-L-arginine methyl ester (TAME) hydrolyzing activities were found in dog urine and one of them was confirmed to be dog urinary kallikrein (DUK, E.C. 3. 4. 21. 8). Mainly by chromatographic techniques, the kallikrein was purified about 1390-fold from the dialyzed dog urine with the overall yield of 36% of vasodilator activity. The specific activities of the finally purified preparation of DUK were 1250 kallikrein units (KU)/A_<280> of vasodilator activity and 11.3μmol/min/A_<280> of TAME esterolytic activity, and the preparation was homogeneous on disc gel electrophoresis and ultracentrifugal analysis. The molecular weight of DUK was estimated to be approximately 2.6 to 2.9×10^4 by vertical plate polyacrylamide gel electrophoresis, gel filtration on a Sephadex G-100 column and ultracentrifugation, and the isoelectric point of this enzyme was also determined as pI 4.2. Various properties such as pH stability, optimum pH for esterolytic activity and heat stability were similar to those of dog renal kallikrein.

• 社団法人日本薬学会の論文
• 1982-07-25

著者

• 守屋 寛

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo

• 松田 兆史

  Faculty of Pharmaceutical Sciences, Science University of Tokyo

• 守屋 寛

  Faculty of Pharmaceutical Sciences, Science University of Tokyo

• 藤本 幸男

  北海道薬科大学臨床生化学教室

• 藤本 幸男

  北海道薬科大学生化学研究室

• 藤本 幸男

  Hokkaido Institute of Pharmaceutical Science

• 宮崎 匡輔

  Faculty of Pharmaceutical Sciences, Science University of Tokyo

• 法島 美男

  Scripps Clinic and Research Foundation

• 宮崎 匡輔

  Faculty Of Pharmaceutical Sciences Science University Of Tokyo

• 守屋 寛

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo

• 守屋 寛

  Faculty Of Pharmaceutical Science Science University Of Tokyo

• 法島 義男

  Faculty Of Pharmaceutical Sciences Science University Of Tokyo:(present Address)department Of Immuno

• 松田 兆史

  明治薬科大学生体機能分析学教室

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