Human Urinary Kallikrein. II. Analysis of Asparagine-Linked Oligosaccharides by Using Lectins(Biological)
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概要
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The patterns of micro-heterogeneity in relation to the carbohydrate structures of active- and latent-types of normal human urinary kallikrein (HUK) were analyzed by serial lectin agarose chromatographies (concanavalin A-, erythroagglutinating phytohemagglutinin-, lentil lectin- and wheat germ agglutinin-agarose chromatographies) and crossed addino-immunoelectrophoresis. In the case of active HUK, the species carrying tri- and/or tetra-aniennary oligosaccharide(s), corefucosylated bi-antennary oligosaccharidc(s), and bi-antennaiy oligosaecharides containing outer galactose residues and an N-acetylglucosamine residue linked β1,4 to a β-linked mannose residue (bisecting N-acetylglucosamine residue) amounted to approximately 36, 33 and 17% of the total active-type HUK, respectively. The micro-heterogencity of the latent-type HUK found by serial lectin affinity ehromatographies was similar to that of the active-type HUK.
- 公益社団法人日本薬学会の論文
- 1987-05-25
著者
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池北 雅彦
東京理科大学 理工学部 応用生物科学科
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池北 雅彦
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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守屋 寛
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
山口東京理大 基礎工
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木付 和幸
Department Of Materials Science And Environmental Engineering Faculty Of Science And Engineering Tok
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守屋 寛
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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池北 雅彦
東京理大
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増永 卓司
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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池北 雅彦
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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増永 卓司
Research Laboratory, KOSE Corporation
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増永 卓司
Research Laboratory Kose Corporation
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木付 和幸
山口東京理科大学、基礎工学部、物質・環境工学科
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