Action of Various Kallikreins and Related Enzymes on Synthetic Arginine and Lysine Derivatives as Substrates

概要

論文の詳細を見る
The substrate specifioities of some glandular and plasma kallikreins, bovine trypsin and plasmin, and boar acrosin were investigated using five tripeptidyl-p-nitroanilides and ten arginine and lysine ester derivatives as substrates. Of the substrates examined, N-α-benzoyl-L-arginine methyl and ethyl esters (Bz-Arg-Me and Bz-Arg-Et) and N-α-acetyl-L-arginine methyl ester (Ac-Arg-Me) were the most effective for the pancreatic and intestinal kallikreins, while N-α-tosyl-L-arginine methyl ester (Tos-Arg-Me) and Bz-Arg-Me were effectives as substrates for the human salivary and guinea-pig coagulating gland kallikreins, respectively. Ac-Arg-Me and N-α-acetyl-glycyl-L-lysine methyl ester (Ac-Gly-Lys-Me) were also good substrates for the boar acrosin. All of the tripeptidyl-p-nitroanilide substrates examined were hydrolyzed relatively ineffeotively by glandular kallikreins and boar acrosin. However, D-valyl-L-leucyl-L-arginine-p-nitroanilide (Val-Leu-Arg-pNA) and D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide (Pro-Phe-Arg-pNA) were found to be moderately useful substrates for various kallikreins and related enzymes.
社団法人日本薬学会の論文
1982-05-25