ラット精巣に存在するアンジオテンシンI-変換酵素の酵素学的性質 : ラット肺酵素との比較検討
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概要
- 論文の詳細を見る
Some properties of angiotensin I-converting enzyme existed in rat testis (RT-ACE) were compared with those of angiotensin I-converting enzyme in rat lungs (RP-ACE). In gel filtration through Sephadex G-200 column, distribution coefficient of RT-ACE (0.34) was higher than that of RP-ACE (0.27). Furthermore, RT-ACE migrated faster than RP-ACE on SDS-polyacrylamide gel electrophoresis. The molecular weights of RT-ACE and RP-ACE were estimated to be 104 and 150 kilodaltons, respectively, on the SDS-polyacrylamide gel electrophoresis. On the other hand, enzymo-chemical properties of both ACEs were very similar when they were assayed by using N^α-hippuryl-His-Leu-OH (HHL) as substrate. Namely, 1) HHL hydrolyzing activities of both ACEs were strongly activated by the addition of NaCl. And the effects of NaCl concentrations on the enzyme activities were similar between RT-ACE and RP-ACE. 2) The effects of various pH on the HHL hydrolyzing activities were also very similar between both ACEs under both conditions where NaCl was present or absent. 3) K_m values for HHL hydrolysis of RT-ACE and RP-ACE were estimated to be the same (2.5 mM) at pH 8.3,in the presence of 0.8 M NaCl. 4) The HHL hydrolyzing activity of RT-ACE was inhibited by the addition of captopril or MK-422,specific inhibitors of angiotensin I-converting enzyme, to similar extent to RP-ACE. From these results, it was speculated that the portions of RT-ACE contributing to the appearance of enzyme activities would resemble those of RP-ACE although the molecule of RT-ACE is different from that of RP-ACE.
- 公益社団法人日本薬学会の論文
- 1985-05-25
著者
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山口 高正
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Since University Of Tokyo
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池北 雅彦
東京理科大学 理工学部 応用生物科学科
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守屋 寛
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
山口東京理大 基礎工
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木付 和幸
Department Of Materials Science And Environmental Engineering Faculty Of Science And Engineering Tok
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木付 和幸
東京理科大学
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守屋 寛
東京理科大学薬学部生化学教室
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守屋 寛
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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池北 雅彦
東京理大
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山口 高正
東京理科大学薬学部, 生化学教室
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関根 雅恵
東京理科大学薬学部, 生化学教室
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関根 雅恵
東京理科大学薬学部 生化学教室
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木付 和幸
山口東京理科大学、基礎工学部、物質・環境工学科
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守屋 寛
東京理科大学薬学部生化学
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守屋 寛
東京理科大学薬学部
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