Porcine Pancreatic Prokallikrein. II. Purification and Some Properties of Prokallikrein A
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概要
- 論文の詳細を見る
The presence of two main components of porcine pancreatic prokallikrein (prokallikreins A and B) was observed on anion-exchange chromatography. One of them, prokallikrein A, was purified by water extraction, followed by a combination of ammonium sulfate fractionation, ion-exchange chromatographies, preparative disc gel electrophoresis and gel filtration in the presence of various protease inhibitors. Prokallikrein A reacted immunologically with the antibody against kallikrein A obtained from autolyzed porcine pancreas. It migrated slightly more slowly than kallikreins A and B on immunoelectrophoresis. The molecular weight of prokallikrein A was estimated to be 3.6-3.9×10^4 by high performance liquid chromatography. On the other hand, those of kallikreins A and B were estimated to be 3.3-3.4×10^4 by the same method. Thus, it was speculated that at least large fragment would not be liberated from the prokallikrein molecule during the activation by protease (s).
- 公益社団法人日本薬学会の論文
- 1982-09-25
著者
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池北 雅彦
東京理科大学 理工学部 応用生物科学科
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鎌田 雅史
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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池北 雅彦
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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守屋 寛
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
山口東京理大 基礎工
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木付 和幸
Department Of Materials Science And Environmental Engineering Faculty Of Science And Engineering Tok
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守屋 寛
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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池北 雅彦
東京理大
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池北 雅彦
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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木付 和幸
山口東京理科大学、基礎工学部、物質・環境工学科
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