Generation of α- and β-Kallikreins from Porcine Panceratic Prokallikrein by the Action of Trypsin

概要

論文の詳細を見る
Prokallikrein was activated into α-kallikrein (single polypetide chain) by the action of trypsin and further converted to β-kallikrein (two polypeptide chains) as indicated by the following observations;1) When prokallikrein was activated with trypsin, rapid generation of kallikrein activity was observed followed by a slow decrease in the kallikrein activity during prologed incubation. The same phenomenon of the slow decreased in the activity of α-kallikrein separately isolated was also observed on trypsin tratment.2) From the results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the kallikrein which was rapidly generated from prokallikrein by the action of trypsin was α-kallikrein. And the kallikrein obtained from α-kallikrein by further treatment with trypsin was β-kallikrein.3) The N-terminal amino acid of α-kallikrein was isolecine. In the case of β-kallikrein, two amino acids, isoleucine and alanine, were detected as N-terminal amino acids.Conversion of α-kallikrein to β-kallikrein by the actioin of trypsion caused a decrease of N^α-benzoyl-L-arginine ethyl ester (Bz-Arg-OEt) hydrolyzing activity and changes of the kinetic constants for the hydrolysis of Bz-Arg-OEt. The kinetic constants of this β-kallikrein were distirictyl different from those of the β-kallikrein obtained from autolyzed pancreas.The above observation suggested that the β-kallikrein obtained in the present paper was a different type of β-kallikrein from that obtained from autolyzed pancreas.
公益社団法人日本薬学会の論文
1988-12-25

Generation of α- and β-Kallikreins from Porcine Panceratic Prokallikrein by the Action of Trypsin

スポンサーリンク

概要

著者

関連論文

スポンサーリンク