コンゴーレッドエラスチンを用いるエラスターゼの改良活性測定法
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概要
- 論文の詳細を見る
An expeditious and sensitive colorimetric method to measure elastase activity using Congo red-elastin as a substrate is described. Highly purified elastase EI as low as 1.65 μg may be assessed accurately within 1 h. This assay is thus 10-100 times more sensitive than previous methods using elastin as a substrate. In this assay method, however, the elastinolytic activity is amplified when a sufficient amount of trypsin and/or chymotrypsin is present in the assay samples. Therefore it is necessary to inhibit the activities of contaminant trypsin and/or chymotrypsin by limabean trypsin inhibitor (LBTI) and chymostatin, by which elastase activity was not influenced. And the coefficients between amidolytic and elastinolytic activities assayed by our methods of both highly purified and crude elastase preparations (AU/CU) were exactly equal to be about 14.
- 社団法人日本薬学会の論文
- 1983-11-25
著者
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池北 雅彦
東京理科大学 理工学部 応用生物科学科
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守屋 寛
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
山口東京理大 基礎工
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木付 和幸
Department Of Materials Science And Environmental Engineering Faculty Of Science And Engineering Tok
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木付 和幸
東京理科大学
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竹本 裕美
Department Of Physiology Ii Hiroshima University Faculty Of Medicine
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守屋 寛
東京理科大学薬学部生化学教室
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守屋 寛
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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椎名 佳子
東京理科大学薬学部・生化学教室
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竹本 裕美
東京理科大学薬学部・生化学教室
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池北 雅彦
東京理大
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木付 和幸
山口東京理科大学、基礎工学部、物質・環境工学科
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守屋 寛
東京理科大学薬学部
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