5. 血中キニン抑制

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Inhibitor, specially acting against human plasma kallikrein (HPK) (both acetone and glass activated), was discovered in potatoes and purified. Sixteen kinds of Japanese and 22 foreign potatoes were examined to screen its content. Purifications were performed by salting out with ammonium sulfate, DEAE-cellulose treatment, CM-cellulose, CM-Sephadex and hydroxyapatite column chromatographies and "Ampholine" electrofocusing method, mainly from the Japanese potato "Danshaku-imo". Its inhibitory action was examined by TAMe esterolytic and dog vasodilative activities as kallikrein determination assays and by rat uterus based on released kinin, after pre-incubation with various kallikreins and other enzymes. Purified material inhibited HPK (acetone activated) and its activation process strongly, HPK (glass activated) qualitatively, human urinary kallikrein (HUK) and hog pancreatic one (Hg Panc K) both slightly, and human salivary one (HSK) and also human plasmin (streptokinase activated) both very slightly. This inhibitor was tremendously more active than Trasylol against HPK (acetone) while it was much weaker than Trasylol against Hg Panc K. This material was kind of neutral protein, molecular weight about near 20,000 and unstable for heating. It was also found that this had potent anti-inflammatory actions on rats produced carragenin edema and CM-C pouch exudate, and also inhibited kinin-formation and edema-producing activity from rat serum when acidification in vitro. It has been indicated from these results that kinin would play in some inflammatory states as one of mediators and this inhibitor blocked such reaction in some extent.
日本アレルギー学会の論文
1969-06-30

5. 血中キニン抑制

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