Porcine Pancreatic Prokallikrein. IV. : N-Terminal Amino Acid Sequences of Prokallikrein B and the Kallikrein Generated from It by the Action Trypsin
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概要
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Porcine pancreatic prokallikreins A and B were separately highly purified by water extraction in the presence of various protease inhibitors, ammonium sulfate salting-out fractionation, anion-exchange chromatography, immunoaffinity chromatography on an anti-porcine pancreatic kallikrein B antibody immobilized Sepharose 4B column and gel filtration on Sephacyl S-200. The specific activities of the final prokallikreins A and B preparations were 75.0 and 79.4 EU/A_<280>(when assayed after activation with trypsin), respectively, and the prokallikrein B preparation showed a single band on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The N-terminal amino acid sequence of prokallikrein B was H-Ala-Pro-Pro- and that of kallikrein B", which is the kallikrein generated from prokallikrein B by the action of trypsin, was H-Ile-Ile-Gly-Gly-. The latter sequence agreed with that of the A-chain of kallikrein B, which is the kallikrein obtained from the autolyzed pancreas.
- 公益社団法人日本薬学会の論文
- 1986-10-25
著者
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鎌田 雅史
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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木付 和幸
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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守屋 寛
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
山口東京理大 基礎工
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木付 和幸
Department Of Materials Science And Environmental Engineering Faculty Of Science And Engineering Tok
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守屋 寛
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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村上 博志
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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平塚 正治
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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平塚 正治
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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村上 博志
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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木付 和幸
山口東京理科大学、基礎工学部、物質・環境工学科
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