Procine Pancreatic Prokallikrein. III. Some Different Forms of Kallikrein Generated from Prokallikrein
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概要
- 論文の詳細を見る
Porcine pancreatic kallikreins generated from their precursors"prokallikreins A and B"in three different ways, i.e., (1) kallikreins A and B obtained from autolyzed pancreas, (2) kallikreins A'and B'spontaneously generated during the purification of prokallikreins A and B and (3) kallikreins A"and B"generated from purified prokallikreins A and B by treatment with trypsin, were each highly purified and their properties were compared. The K_m values for BzArgOEt hydrolysis, the mobilities on immunoelectrophoresis and the elution profiles from a DEAE-Sepharose CL-6B column of kallikreins A'and B'and kallikreins A"and B"closely resembled each other but apparently differed from those of kallikreins A and B. Kallikreins A and B were confirmed to consist of two polypeptide chains (three chains to some extent), while kallikreins A", B"and B'were each determined to consist of a single chain. The amino acid compositions of the two-chain kallikrein and the single-chain kallikrein were very similar, though somewhat higher values of Leu, Glu and Lys residues were observed in the single-chain kallikrein as compared with the two-chain kallikrein. Thus, kallikreins A"and B"generated from prokallikreins A and B by the action of trypsin were considered to be very similar to, or identical with, kallikreins A'and B'spontaneously generated during the purification of prokallikreins A and B. It is speculated that two-chain kallikreins A and B are generated from single-chain kallikreins A"and B"by the action of some protease (s) other than trypsin during the autolysis process.
- 公益社団法人日本薬学会の論文
- 1984-09-25
著者
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池北 雅彦
東京理科大学 理工学部 応用生物科学科
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鎌田 雅史
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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池北 雅彦
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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守屋 寛
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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木付 和幸
山口東京理大 基礎工
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木付 和幸
Department Of Materials Science And Environmental Engineering Faculty Of Science And Engineering Tok
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守屋 寛
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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池北 雅彦
東京理大
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滝口 宏子
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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池北 雅彦
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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滝口 宏子
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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木付 和幸
山口東京理科大学、基礎工学部、物質・環境工学科
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