Studies on the Passage of α-Chymotrypsin across the Intestine. II. Enzymic Activity and Radioactive Macromolecule recovered in the Mesenteric Perfusate
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概要
- 論文の詳細を見る
A perfusion with Krebs-Ringer solution containing dextran through the mesenteric vascular system was performed on rat to which ^<131>I-labelled α-chymotrypsin was administered in the jejunum. This method sufficed for the purpose of eliminating various blood components, such as the inhibitors for the enzyme, which interfer with quantitative assay of a small amount of the enzyme absorbed. Using the method, the non-diffusible ^<131>I-bound substance (s) and N-acetyl-L-tyrosine ethyl ester (ATEE)-hydrolyzing activity were found in the mesenteric perfusates of the rats, and the amount of the enzyme absorbed during 90 min was estimated to be about 0.6% of the infused dose. Furthermore, when the immunoreactivity with the antiserum against α-chymotrypsin was examined on the mesenteric perfusate, a precipitin band sharply indicating the enzyme was formed with the perfusate collected during the first 30 min after the administration. These results strongly suggest that small but significant amount of α-chymotrypsin is transmitted across the intestinal wall into the mesenteric vascular system as retaining its enzymic and immunochemical properties.
- 社団法人日本薬学会の論文
- 1974-05-25
著者
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山口 稽子
東京理科大学薬学部
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森脇 千秋
Laboratory Of Physiological Chemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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守屋 寛
Laboratory of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tok
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山口 稽子
Laboratory of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tok
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森脇 千秋
Laboratory of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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