Studies on Proteinases from Ficus carica var. HORAISHI. VI. Immunochemical Comparison of Ficins A, B, C, D and S
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概要
- 論文の詳細を見る
To clarify the multiplicity of proteinases, i.e. Ficins A, B, C, D, and S (EC 3.4.4.12) from Ficus carica var. HORAISHI, immunochemical properties of the enzymes were examined. Each enzyme used was proved to be homogeneous with immunoelectrophoresis. Antibodies were purified from rabbit antisera by ammonium sulfate fractionation, the use of Ficins immunoadsorbents and Sephadex G-200 gel-filtration. The relation of the enzymes was examined with Ouchterlony's double immunodiffusion, immunoelectrophoresis, cross-reaction among the enzymes on an immunoadsorbent column and inhibition of the caseinolytic activity of the enzymes by the antibodies. The results from the experiments are as follows : (a) Ficins A and S were immunochemically identical and also Ficins B and C resembled to each other. (b) Ficins A and S, Ficin B, Ficin C, and Ficin D possessed common antigenic determinants at the rate of more than 38%, but the enzymes could be distinguished, in case that the relation of Ficin A and Ficin S be excepted. From these results, it is suggested that the immunochemical similarity of the enzymes to Ficin A is in the order of Ficin A=Ficin S>Ficin B>Ficin C>Ficin D.
- 社団法人日本薬学会の論文
- 1975-09-25
著者
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佐々木 正憲
Niigata College Of Pharmacy
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山口 稽子
東京理科大学薬学部
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森脇 千秋
Laboratory Of Physiological Chemistry Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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佐々木 正憲
Department of Pharmacy, Tokyo College of Pharmacy
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山口 稽子
Laboratory of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tok
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佐々木 正憲
Department Of Pharmacology And Pharmacy Niigata College Of Pharmacy
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森脇 千秋
Laboratory of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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