Pharmaceutical Studies on Aminopeptidase from Aspergillus japonica. I
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概要
- 論文の詳細を見る
To utilize aminopeptidase from Aspergillus japonica as a digestive enzyme preparation, the properties of the crude enzyme were examined from pharmaceutical aspects. The enzyme was most active at pH 8.0 and 50°, and was stable in a pH range of 5.5 to 8.5 and below 50°. The enzyme was activated by Co^<2+>, but was strongly inhibited by some metal ions, ophenanthroline, and N-bromosuccinimide. Hydrolytic cross-reaction between the enzyme and various proteases was carried out using casein as a substrate. The hydrolysis ratio (60%) was 2-fold greater than that by only the individual proteases. Therefore, the enzyme is considered to be useful for digesting a protein, together with other proteases. From the examination of the stability of the enzyme powder and that of the enzyme to various pharmaceutical factors, the enzyme was found to be highly stable to diluents, binders, and wetting agents at a high concentration, and was also stable to low first pressure in tabletting. From these results, it is suggested that this aminopeptidase can be satisfactorily utilized as a digestive enzyme preparation.
- 公益社団法人日本薬学会の論文
- 1976-10-25
著者
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杉浦 衛
Department of Pharmacy, Tokyo College of Pharmacy
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佐々木 正憲
Niigata College Of Pharmacy
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佐々木 正憲
Department of Pharmacy, Tokyo College of Pharmacy
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石川 正夫
東京薬科大学
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鈴木 睦子
東京薬科大学
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鈴木 睦子
Department of Pharmacy, Tokyo College of Pharmacy
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石川 正夫
Department of Pharmacy, Tokyo College of Pharmacy
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佐々木 正憲
Department Of Pharmacology And Pharmacy Niigata College Of Pharmacy
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